Effect of matrix glycation on expression of type IV collagen, MMP-2, MMP-9 and TIMP-1 by human mesangial cells

Cell Adhesion and Communication
S S AndersonE C Tsilibary

Abstract

Human mesangial cells grown in either 5 or 25 mM glucose were cultured on type IV collagen which had been previously control-incubated or in vitro glycated. Northern blot analysis revealed that after 3-7 days in culture mesangial cells on glycated type IV collagen expressed approximately 25-200% more alpha 1 (IV), approximately 20-50% less matrix metalloproteinase 2 (MMP-2), and 65-75% more tissue inhibitor of metalloproteinase 1 (TIMP-1). Decreased immunoreactivity (approximately 30-40%) and collagenolytic activity (approximately 10-40%) corresponding to MMP-2 was also detected in media conditioned during the third day of culture on glycated type IV collagen. These effects on cell function were related to the extent of type IV collagen modification and were similar for cells cultured in 5 or 25mM glucose. Elevated glucose (25 vrs 5 mM) increased expression of alpha 1 (IV) mRNA (approximately 40-70%) and in conjunction with matrix glycation resulted in detectable levels of MMP-9 message by northern blot although collagenolytic activity corresponding to MMP-9 was not detectable by zymography. We conclude that glucose and matrix glycation may each alter mesangial cell function, perhaps leading to an imbalance in mesangial matrix ...Continue Reading

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Citations

Jul 12, 2005·Diabetes Research and Clinical Practice·Seung Won LeeKwan-Woo Lee
Oct 27, 2001·Diabetic Medicine : a Journal of the British Diabetic Association·P R MaxwellD Gordon
Feb 19, 2008·Cardiovascular Research·Gaia SpinettiPaolo Madeddu
May 25, 2007·Connective Tissue Research·Paul E NormanJonathan Golledge
Jul 8, 2003·The Journal of Pathology·Effie C Tsilibary
Jul 18, 2014·Journal of Biomechanical Engineering·Dannielle S FigueroaAlisa Morss Clyne
Oct 3, 2018·International Journal of Oncology·Shen SunDian-Shuai Gao
Mar 7, 2003·American Journal of Physiology. Renal Physiology·Paraskevi V KitsiouEffie C Tsilibary
Mar 16, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Paul VoziyanBilly Hudson
Aug 2, 2003·The British Journal of Dermatology·S J WallG Murphy
Jan 12, 2019·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O N Poteryaeva, I F Usynin
Aug 15, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Panagiotis M KaramessinisEffie C Tsilibary
Aug 8, 2002·The Journal of Investigative Dermatology·Steven J WallGillian Murphy

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