PMID: 15332998Aug 31, 2004Paper

Effect of medium on chromatin damage in bystander mammalian cells

Radiation Research
M SuzukiTom K Hei

Abstract

In the present study, we examined the potential contribution of irradiated medium to the bystander effect using custom-made double-Mylar stainless steel rings. Exponentially growing human-hamster hybrid (A(L)) cells were plated on either one or both sides of double-Mylar dishes 2-4 days before irradiation. One side (with or without cells) was irradiated with alpha particles using the track segment mode of a 4 MeV Van de Graaff accelerator at the Radiological Research Accelerator Facility of Columbia University. Since alpha particles can traverse only a very limited distance (around 23 microm in water), cells plated on the other side of a medium-filled Mylar dish will not be irradiated by the alpha particles. The results of the cytogenetic assay of unirradiated target cells that were attached to the top Mylar layer indicate that the number of chromatid-type aberrations was higher when there was a bottom layer of cells in the medium-filled chambers than with just medium alone. Furthermore, when the medium was transferred from these cell-irradiated dishes to fresh A(L) cell cultures, chromatid-type aberrations were produced in the unirradiated fresh cells. In contrast, medium irradiated in the absence of cells had no effect on chr...Continue Reading

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Citations

Apr 29, 2005·Uchū Seibutsu Kagaku·Ken Ohnishi, Takeo Ohnishi
Sep 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Oleg V BelyakovDavid J Brenner
Feb 13, 2010·International Journal of Radiation Biology·Marianne B SowaWilliam F Morgan
Jul 19, 2006·International Journal of Radiation Biology·Fiona M LyngKevin M Prise
Mar 15, 2006·Cell Communication & Adhesion·Ferya Banaz-YaşarAlexandra Gellhaus
Jul 23, 2008·The Journal of Pharmacy and Pharmacology·Tom K HeiCharles R Geard
Jul 23, 2008·The Journal of Pharmacy and Pharmacology·Kim L ChapmanMunira A Kadhim
Jul 7, 2011·Dose-response : a Publication of International Hormesis Society·Bobby E LeonardGeorgia C Beecher
Jan 2, 2015·Dose-response : a Publication of International Hormesis Society·A BelchiorP Almeida
Mar 10, 2005·International Journal of Cancer. Journal International Du Cancer·Chunlin ShaoKevin M Prise
Jan 4, 2008·Environmental and Molecular Mutagenesis·Olga Kovalchuk, Janet E Baulch
Nov 22, 2005·Radiation Research·Rong Wang, Jeffrey A Coderre
Mar 10, 2009·Radiation Research·Hatim FakirRainer K Sachs
Jan 14, 2009·Radiation Research·Torsten GroesserBjorn Rydberg
Jul 8, 2009·Radiation Research·Asima ChakrabortyRobert W Redmond
Mar 1, 2007·Journal of Radiation Research·Nobuyuki HamadaYasuhiko Kobayashi
Mar 16, 2007·International Journal of Radiation Biology·Yuichi KanasugiKaoru Takakura

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