Effect of medium sugar source on the production of retroviral vectors for gene therapy

Biotechnology and Bioengineering
Ana S CoroadinhaManuel J T Carrondo

Abstract

The production of gene therapy retroviral vectors presents many difficulties, mainly due to vector instability and low cell productivities hampering the attainment of high titers of infectious viral vectors. The objective of this work is to increase the production titers of retroviral vectors by manipulating the sugar carbon sources used in bioreaction. Four sugars were tested (glucose, galactose, sorbitol, and fructose) on an established Moloney murine leukemia virus (MoMLV) producer cell line. Galactose and sorbitol did not support cell growth or vector production. Glucose supplemented at 25 mM supported the highest cell growth; however, the use of glucose or fructose at 83 and 140 mM have shown to improve the infectious vector titer three to fourfold. The reasons for the titer improvements were further analyzed and, although, the cell-specific productivity in viral transgene RNA and reverse transcriptase were augmented 5- and 6-fold for glucose at 140 mM and 14- and 16-fold for fructose at 140 mM, comparing with glucose at 25 mM, these increases did not seem sufficient to account for the 14- (140 mM glucose) and 32- (140 mM fructose) fold increment obtained for the infectious particles-specific productivity. Further accounti...Continue Reading

References

Apr 1, 1978·Journal of Cellular Physiology·H R ZielkeM Cornblath
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H R ZielkeM Cornblath
Jan 1, 1990·Methods in Enzymology·P Matsudaira
Oct 1, 1991·Physiological Reviews·A Garcia-Perez, M B Burg
Aug 1, 1989·Experimental Cell Research·C WolfromM Gautier
Apr 1, 1988·Journal of Cellular Physiology·K W Lanks, P W Li
Jul 8, 1980·Biochimica Et Biophysica Acta·I SussmanD F Wilson
Sep 1, 1980·Journal of Cellular Physiology·H R ZielkeP T Ozand
Apr 1, 1996·Applied Biochemistry and Biotechnology·D Petch, M Butler
May 1, 1996·Applied Microbiology and Biotechnology·S G LeeB G Kim
Mar 10, 1998·Endocrinology·A E BuchsE Cerasi
Jul 9, 1999·Biotechnology and Bioengineering·J M Le DouxM L Yarmush
Aug 22, 2000·The Journal of General Virology·M W YapA J Kingsman
Sep 8, 2001·American Journal of Physiology. Cell Physiology·D Y Hwang, F Ismail-Beigi
Jan 1, 1997·Journal of Biomedical Science·H.B. Rasmussen
Dec 18, 2001·Biotechnology and Bioengineering·C AltamiranoF Gòdia
Apr 5, 2003·Biotechnology Progress·Kaiming YeJerome S Schultz
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Apr 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·V J CRISTOFALO, D KRITCHEVSKY
Feb 24, 2004·The Journal of Gene Medicine·O-W Merten
May 26, 2004·Journal of Virological Methods·M CarmoM J T Carrondo
Apr 25, 1991·Biotechnology and Bioengineering·S S Ozturk, B O Palsson
Jun 20, 1995·Biotechnology and Bioengineering·S K OhA B Choo

❮ Previous
Next ❯

Citations

Apr 7, 2006·Applied Microbiology and Biotechnology·A S CoroadinhaM J T Carrondo
Jan 5, 2011·Cytotechnology·Yoshinobu KonnoShinji Hosoi
Jan 19, 2008·Cancer Gene Therapy·C H J LamersJ W Gratama
Sep 1, 2012·Human Gene Therapy Methods·Vanessa BandeiraManuel J T Carrondo
Apr 18, 2013·Expert Opinion on Biological Therapy·Maria Mercedes SeguraAlain Garnier
Sep 17, 2008·Journal of Biotechnology·Ana I AmaralPaula M Alves
Jun 10, 2009·The Journal of Gene Medicine·Marlene CarmoPedro Estilita Cruz
Dec 3, 2014·Trends in Biotechnology·Ana F RodriguesAna S Coroadinha
May 17, 2011·Vaccine·Ana Carina SilvaPaula M Alves
Jan 22, 2018·Biotechnology Letters·Hugo R SoaresAna S Coroadinha
May 24, 2015·Applied and Environmental Microbiology·Malak A EsseiliQiuhong Wang
Aug 28, 2012·Current Opinion in Endocrinology, Diabetes, and Obesity·Ava M PortNawfal W Istfan

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.