Effect of melatonin supplementation in the long-term preservation of the sheep ovaries at different temperatures and subsequent in vitro embryo production.

Theriogenology
Abbas GoodarziNavid Dadashpour Davachi

Abstract

Investigations in the past decades have shown that oocytes developmental competence following in vitro fertilization is greatly influenced by an interval between isolation of the ovaries immediately after death/slaughter and oocytes recovery from the visible follicles. In order to determine the optimal conditions for long-term preservation of ovaries, an experiment was conducted with adding different doses of melatonin (0 (C), 500 (M1), 600 (M2), 700 (M3) and 800 (M4) μM) as an antioxidant to sheep ovaries preservation medium (PBS) maintained at 4 and 20 °C for 24 h. The effects on in vitro embryo production (IVEP) parameters including maturation, fertilization, cleavage, and blastocyst rates and the total number of blastomere were evaluated after the ovaries preservation. Melatonin reduced the decline in fertilization rate as an indicator of success in vitro maturation (P ≤ 0.05). Furthermore, ovarian storage time had significant negative effect (P ≤ 0.05) on IVEP parameters. Supplementation with melatonin increased the total cell number of blastocysts as an indicator of embryo quality (i.e. mean blastomeric cells in 4°C groups: 86.00 ± 3.00, 98.50 ± 3.5, 111.5 ± 1.5, 125.5 ± 2.00 and 126.50 ± 5.5 for C, M1, M2, M3 and M4. res...Continue Reading

Citations

Aug 28, 2020·Frontiers in Molecular Biosciences·Tie Cheng SunLu Yang Cheng
Sep 5, 2020·Journal of Assisted Reproduction and Genetics·Janice de M V VilelaChristiani A Amorim
May 1, 2020·Animals : an Open Access Journal From MDPI·Irene Sánchez-AjofrínAna Josefa Soler
Jan 1, 2018·Reproduction, Fertility, and Development·José-Alfonso AbeciaAdriana Casao
Dec 9, 2020·Reproductive Biomedicine Online·Janice de Miranda Vasconcellos VilelaChristiani Andrade Amorim
Dec 23, 2020·Animals : an Open Access Journal From MDPI·Alicia Martín-MaestroAna Josefa Soler
Aug 2, 2020·Cryobiology·Xiao Chao LiuSong He Yang

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