PMID: 38843Aug 7, 1979

Effect of membrane potential and internal pH on active sodium-potassium transport and on ATP content in high-potassium sheep erythrocytes

Biochimica Et Biophysica Acta
A M Zade-OppenD C Tosteson

Abstract

Ouabain-sensitive Na+ and K+ fluxes and ATP content were determined in high potassium sheep erythrocytes at different values of membrane potential and internal pH. Membrane potential was adjusted by suspending erythrocytes in media containing different concentrations of MgCl2 and sucrose. Concomitantly either the external pH was changed sufficiently to maintain a constant internal pH or the external pH was kept constant with a resultant change of internal pH. The erythrocytes were preincubated before the flux experiment started in a medium which produced increased ATP content in order to avoid substrate limitation of the pump. It was found that an increased cellular pH reduced the rates of active transport of Na+ and K+ without significantly altering the ratio of pumped Na+/K+. This reduction was not due to limitation in the supply of ATP although ATP content decreased when internal pH increased. Changes of membrane potential in the range between -10 and +60 mV at constant internal pH did not affect the rates of active transport of Na+ or K+.

References

Aug 1, 1988·The Journal of Membrane Biology·A M Zade-OppenD C Tosteson
May 8, 2004·American Journal of Physiology. Renal Physiology·Karl S LangChristophe Duranton

Citations

Nov 15, 1977·Biochimica Et Biophysica Acta·J C Ellory, P Maher
Apr 21, 1975·Biochimica Et Biophysica Acta·L A BeaugeO Ortiz
Jan 1, 1974·Annals of the New York Academy of Sciences·F J Brinley, L J Mullins
Sep 1, 1967·The Journal of Physiology·P J Garrahan, I M Glynn
Jul 11, 1973·Nature: New Biology·A Cass, M Dalmark
Jan 1, 1970·Biochimica Et Biophysica Acta·K K Tsuboi, K Fukunaga
Sep 1, 1966·British Heart Journal·P SaltzmanA Pick
Dec 1, 1959·The Journal of Physiology·V BOLINGBROKE, M MAIZELS
Jan 1, 1965·Transfusion·E BEUTLER, O DURON
Jan 1, 1965·The Journal of General Physiology·P HOROWICZ, C J GERBER
Jan 1, 1965·The Journal of General Physiology·P HOROWICZ, C J GERBER
Jan 1, 1959·Acta Obstetricia Et Gynecologica Scandinavica·J NAESLUNDB LARSSON
Mar 20, 1942·The Journal of General Physiology·M H Jacobs, D R Stewart
Dec 1, 1969·The Journal of Membrane Biology·J A Donlon, A Rothstein

Related Concepts

Resting Potentials
Potassium
Dall Sheep
Ouabain
Magnesium Chloride
Sodium
Sheep antigen
Specimen Type - Erythrocytes
Alkalemia
Domestic Sheep

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