Effect of mercaptoethanol in the radioactive thin layer chromatography assay of NAD+-15-hydroxyprostaglandin in dehydrogenase

Prostaglandins
J T Wright, C N Corder

Abstract

The use of mercaptoethanol in the assay of rat kidney 15-hydroxyprostaglandin dehydrogenase (PGDH) was found to have minimal effect on activity assayed with the spectrophotometric and substrate loss assays. However, mercaptoethanol appeared to inhibit PGDH when assayed by thin-layer chromatography, based upon conversion of 3H-PGE1 to 15-keto-3H-PGE1. Mercaptoethanol reacted with 15-keto-PGE1 to alter its chromatographic mobility and to suppress the U.V. absorption spectrum of 15-keto-PGE1. The implication of the use of ME in radiometric assays is discussed.

Citations

Oct 1, 1976·Prostaglandins·H S Hansen
Dec 17, 1969·Biochimica Et Biophysica Acta·H VonkemanD A van Dorp

Related Concepts

2-Mercaptoethanol
Hydroxyprostaglandin Dehydrogenases
Sputolysin
Kidney
Chromatography, Qualitative; Thin Layer, Analyte Not Elsewhere Specified
PHGDH wt Allele
Hpgd
NADP
Prostaglandin E1 Measurement
15-Hydroxyprostaglandin dehydrogenase (NAD+)

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