Effect of mercury on aryl hydrocarbon receptor-regulated genes in the extrahepatic tissues of C57BL/6 mice

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Issa E A AmaraAyman O S El-Kadi

Abstract

The individual toxic effects of aryl hydrocarbon receptors (AhR) ligands such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or heavy metals typified by mercury (Hg(2+)) has been previously demonstrated. However, little is known about the combined toxic effects of TCDD and Hg(2+)in vivo. Therefore, we examined the effect of exposure to Hg(2+) (2.5mg/kg) in the absence and presence of TCDD (15 μg/kg) on the AhR-regulated genes using C57Bl/6 mice. Hg(2+) alone did not affect kidney, lung, or heart Cyp1a1/1a2/1b1 mRNA levels. On the contrary, Hg(2+) alone significantly induced kidney Cyp1a1/1a2/1b1 and lung Cyp1b1 protein and catalytic activities. Hg(2+) also induced Nqo1, Gsta1, and HO-1 at the mRNA, protein, and activity levels in the kidney and heart but not in the lung. Upon co-exposure to Hg(2+) and TCDD, Hg(2+) significantly potentiated the TCDD-mediated induction of kidney and lung Cyp1a1/1a2/1b1 mRNA levels, while it decreased their kidney protein and catalytic activity and it increased their lung protein. In addition, Hg(2+) potentiated the TCDD-mediated induction of Nqo1, Gsta1, and HO-1 at mRNA, protein and activity levels in all tissues. The present study demonstrates that Hg(2+) modulates the constitutive and TCDD-indu...Continue Reading

References

Jan 1, 1991·Free Radical Biology & Medicine·P C PreuschD Ross
Aug 30, 1994·Biochemical Pharmacology·M D BurkeR T Mayer
Aug 26, 1998·Toxicology and Applied Pharmacology·J SundbergA Oskarsson
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 21, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·David C SpinkLaurence S Kaminsky
May 30, 2003·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Masuhiro NishimuraTetsuo Satoh
Aug 14, 2003·Ecotoxicology and Environmental Safety·Michael Gochfeld
Oct 24, 2003·Archives of Toxicology·Miroslav MachalaLenka Skálová
Aug 12, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hesham M Korashy, Ayman O S El-Kadi
Nov 13, 2007·Free Radical Biology & Medicine·Shigeyuki UnoDaniel W Nebert
Aug 30, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Beshay N M ZordokyAyman O S El-Kadi
Apr 18, 2009·American Journal of Respiratory Cell and Molecular Biology·Patrick S WongFumio Matsumura
Oct 3, 2009·Toxicology and Applied Pharmacology·Beshay N M ZordokyAyman O S El-Kadi
Aug 17, 2011·Free Radical Biology & Medicine·Issa E A Amara, Ayman O S El-Kadi

❮ Previous
Next ❯

Citations

Jan 1, 2012·Microbial Ecology in Health and Disease·Olav Albert Christophersen
Dec 13, 2012·Toxicology and Applied Pharmacology·Issa E A AmaraAyman O S El-Kadi
May 5, 2017·JCI Insight·Maria Serena LonghiSimon C Robson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.