Effect of metabolic state on phytohemagglutinin-P agglutination of normal human erythrocytes.

Biochimica Et Biophysica Acta
J A Singer, M Morrison

Abstract

A reproducible quantitative assay for the lectin-mediated agglutination of human erythrocytes, depending on different rates of settling of agglutinated and nonagglutinated erythrocytes, was developed. This assay was used to study the aggregation of human erythrocytes by phytohemagglutinin-P. The aggregation of human erythrocytes by phytohemagglutinin-P was found to depend upon the metabolic state of the cells. Metabolically depleted erythrocytes agglutinated much less readily than did similar cells supplied with adenosine. This was not due to swelling and rigidity of the cells, since erythrocytes in hypotonic solution did not exhibit significantly altered phytohemagglutinin-P agglutination. Metabolically depleted erythrocytes, or erythrocytes from blood stored 8 weeks, lysed and resealed in the presence of ATP, were agglutinated by phytohemagglutinin-P to a much greater extent than control samples without ATP. The presence of Mg2+, either alone or with ATP, had little effect on the agglutinability of the resealed membranes. Low concentrations of Ca2+ (0.2 mM) had little effect on agglutinability, although high Ca2+ (5 mM) inhibited agglutinability of the resealed membranes somewhat. Both metabolically depleted erythrocytes and ...Continue Reading

References

Dec 28, 1973·Biochimica Et Biophysica Acta·R L Juliano
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·I VlodavskyL Sachs
Oct 1, 1974·The Journal of Cell Biology·M C Willingham, I Pastan
Oct 18, 1972·Nature: New Biology·G L Nicolson
Jul 1, 1974·The Journal of Cell Biology·T L Steck

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