Effect of metal ion concentration on a biological reactor

Biotechnology and Bioengineering
P G IbbotsonM S Otterburn

Abstract

Saccharomyces cerevisiae (yeast) cells were employed as a source of alcohol dehydrogenase in the NAD(+)-to-NADH reaction. The cells were immobilized in calcium alginate monofilament fibers and used in a biological reactor. The alginate could not be heat sterilized since temperatures above 80 degrees C caused the polymer chains to degrade. The same proved true for the high pH necessary for the reaction, but the alginate strength was increased by Ba(2+) solution treatment. X-ray probe analysis showed that about 30% of the Ca(2+) sites exchanged with the Ba(2+) ions. The Ba(2+) ions (as well as the Ca(2+) ions) permeabilized the cells and increased the reaction rate. Long term trials showed that Ba(2+) ions were slowly elutriated from the fiber biocatalyst, causing a drop in reaction rate. The trend certainly was reversible as far as the fiber was concerned. It is assumed that the permeabilization of the cells by the Ba(2+) ions was a reversible process.

References

Oct 1, 1978·Analytical Biochemistry·G F MiozzariR Hütter
Sep 1, 1987·British Journal of Sports Medicine·S H Ibbotson
Jan 1, 1974·Biomaterials, Medical Devices, and Artificial Organs·E Denti
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Feb 11, 1983·Science·A M Klibanov

Related Concepts

Alcohol dehydrogenase
Barium
Clinical Trials
Ions
Polymers
Saccharomyces cerevisiae
Diagnostic Radiologic Examination
Yeasts
Alginate
Calcium Alginate

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