PMID: 28502303May 16, 2017Paper

Effect of metformin on proliferation and apoptosis of rat prolactinoma MMQ cells and related mechanisms

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Kai JinGang Yang

Abstract

Objective To investigate the effect of metformin on the cell proliferation, cell cycle and apoptosis of rat prolactinoma MMQ cells in vitro and related molecular mechanisms. Methods The MMQ cells were treated with 1.25, 2.5, 5, 10, 20 mmol/L metformin for 48 hours. CCK-8 assay was used to assess the cell proliferation ability; flow cytometry was used to analyze the cell cycle distribution and apoptosis; Western blotting was performed to detect the expressions of AMPKα1/2, p-AMPKα, mTOR, p-mTOR, insulin like growth factor 1 receptor (IGF-1R), ERK1/2, p-ERK1/2, AKT, p-AKT, P21, CDK4, cyclin D1, caspase-3, cleaved caspase-3(c-caspase-3), Bcl-2 and BAX. Results Compared with the control group, metformin inhibited cell proliferation, induced cell cycle arrest in the G0/G1 phase and promoted cell apoptosis in MMQ cells. The expressions of P21 and c-caspase-3 increased, meanwhile, the expressions of CDK4, cyclin D1, caspase-3 and Bcl-2 decreased by metformin. Besides, the expression of p-AMPKα was elevated, but p-mTOR was reduced. Furthermore, the expressions of IGF-1R, p-AKT and p-ERK descended after metformin treatment. Conclusion Metformin could inhibit cell proliferation, induce cell cycle arrest and apoptosis in MMQ cells by acti...Continue Reading

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