Effect of methodology, dilution, and exposure time on the tuberculocidal activity of glutaraldehyde-based disinfectants.

Applied and Environmental Microbiology
E C ColeD J Weber

Abstract

The Association of Official Analytical Chemists (AOAC) test for assessing the tuberculocidal activity of disinfectants has been shown to be variable. A modified AOAC test, which substituted Middlebrook 7H9 broth as the primary subculture medium and used neutralization by dilution, was compared with the standard AOAC method to assess the mycobactericidal activity of three glutaraldehyde-based disinfectants at 20 degrees C and various exposure times. These changes had a marked effect on results, with the modified AOAC test providing more positive penicylinders per 10 replicates in 12 of the 13 comparisons that provided positive results. These differences were observed with both Mycobacterium bovis (ATCC 35743) and a clinical isolate of Mycobacterium tuberculosis. The effects of various exposure times to and dilutions of the glutaraldehyde-based disinfectants were also examined. The minimum exposure time needed to inactivate reliably M. bovis or M. tuberculosis with 2% glutaraldehyde was 20 min at 20 degrees C. Diluting 2% glutaraldehyde caused a significant decline in mycobactericidal activity. Modification of the standard AOAC test to improve its sensitivity in detecting the failure of disinfectants to inactivate mycobacteria is...Continue Reading

References

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Citations

Mar 3, 2004·Diseases of the Colon and Rectum·Douglas B NelsonUNKNOWN Shea
May 12, 2011·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Bret T PetersenUNKNOWN Society for Healthcare Epidemiology of America
Jul 31, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Douglas B NelsonUNKNOWN Society for Healthcare Epidemiology of America
Jan 9, 2010·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·William A RutalaUNKNOWN Society for Healthcare Epidemiology of America
Nov 1, 1994·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·A D Russell
Jun 1, 1999·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·A W GregoryR A Robison
Oct 1, 1990·Journal of Clinical Microbiology·M BestM E Kennedy
Sep 16, 1991·The American Journal of Medicine·W A RutalaD J Weber
Feb 15, 2011·Infectious Disease Clinics of North America·William A Rutala, David J Weber

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