PMID: 12770306May 29, 2003Paper

Effect of methoprene and 20-hydroxyecdysone on vitellogenin production in cultured fat bodies and backless explants from unfed female Dermacentor variabilis

Journal of Insect Physiology
N SankhonL B Coons

Abstract

The effect of 20-hydroxyecdysone (20HE) and the juvenile hormone analogue methoprene (JHA) on vitellogenin (Vg) production in fat body organ cultures and backless explants of unfed female Dermacentor variabilis was measured. An indirect double antibody enzyme linked immunosorbent assay (ELISA) was developed using a monoclonal antibody that recognized a 98 kDa subunit of Vg and a Vg specific polyclonal antibody made against vitellin (Vn). Peak Vg titers in culture medium from fat body cultures treated with 0.1 &mgr;M 20HE or 1 &mgr;M 20HE were 24 ng/ml and 20 ng/ml respectively. In culture medium from backless explants treated with 0.1 &mgr;M 20HE or 1 &mgr;M 20HE, peak Vg titers were 36 ng/ml and 26 ng/ml, respectively. JHA produced only a slight increase in Vg titers that was statistically different from Vg titers produced by 20HE but was not statistically different from hormone-free controls. These results support the conclusion that Vg production in fat body trophocytes of D. variabilis is regulated by 20HE.

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·X X ZhuE M Dotson
Jan 1, 1990·Experimental & Applied Acarology·L B CoonsL Starr-Spires
Apr 22, 1997·Proceedings. Biological Sciences·L O LomasH H Rees
Jul 1, 1997·Insect Biochemistry and Molecular Biology·A M James, J H Oliver

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Citations

May 29, 2003·Journal of Insect Physiology·N P. GudderraR M. Roe
May 29, 2003·Journal of Insect Physiology·Kevin J. Friesen, W Reuben Kaufman
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gro V AmdamRobert E Page
Apr 10, 2012·Experimental Parasitology·Gislaine Cristina RomaMaria Izabel Camargo-Mathias
Aug 25, 2009·Journal of Insect Physiology·Ana R CabreraR Michael Roe
Jul 19, 2008·Journal of Insect Physiology·Adriana SeixasW Reuben Kaufman

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