Effect of methotrexate on collagen-induced arthritis assessed by micro-computed tomography and histopathological examination in female rats

Biomolecules & Therapeutics
Young Hee Kim, Jin Seok Kang

Abstract

We tested the hypothesis that micro-computed tomography (micro-CT) analysis provides a better quantitative readout of the therapeutic potential of methotrexate (MTX) for treating collagen-induced arthritis (CIA) in rats and compared to conventional histopathological examination. Rats were divided into three groups: Group 1 (G1) was treated with 0.9% saline, whereas groups 2 (G2) and 3 (G3) were boosted with type II collagen at days 0 and 7. Following the first collagen immunization, rats in G1 and G2 were treated with 0.9% saline and those in G3 were treated with 1.5 mg/kg MTX from day 14 to 28. All rats were sacrificed on day 28, at which point and all hind knee joints were analyzed by micro-CT and histopathological examination. Micro-CT analyses showed that bone volume and trabecular number were significantly decreased in G2 and G3 compared to G1 (p<0.01), as was percent bone volume (p<0.05 and p<0.01, respectively). However, bone surface/bone volume was significantly increased in G2 and G3 compared to G1 (p<0.05 and p<0.01, respectively). Trabecular separation was significantly increased in G3 compared to G1 (p<0.05). Histopathological examination showed that knee joints of rats in G2 and G3 showed severe joint destruction w...Continue Reading

References

Jan 1, 1997·Life Sciences·L K MyersA H Kang
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Jun 5, 2007·Nature Protocols·David D BrandEdward F Rosloniec
Nov 16, 2007·Arthritis Research & Therapy·Miguel Otero, Mary B Goldring
Feb 22, 2008·Current Pharmaceutical Biotechnology·Hironari TakaishiJeanine D'Armiento
Aug 27, 2009·Methods : a Companion to Methods in Enzymology·Sebastian J SchambachMarc A Brockmann
Sep 28, 2010·Lancet·David L ScottTom W J Huizinga
Dec 15, 2011·Clinical Pharmacology and Therapeutics·D L Scott
Jul 31, 2012·Autoimmunity Reviews·Helena CarvalheiroM Margarida Souto-Carneiro
Dec 12, 2012·The American Journal of Pathology·Wade KobaEugene J Fine
Apr 18, 2014·Nature Reviews. Cancer·James R W ConwayPaul Timpson

❮ Previous
Next ❯

Citations

Jan 16, 2016·Immunopharmacology and Immunotoxicology·Samra Sardar, Åsa Andersson
Aug 25, 2015·European Journal of Pharmacology·Mariam Gamal Fahmy WahbaAli Ahmed Abo-Saif
Sep 29, 2018·Biomarker Insights·Leroy ShervingtonAmal Shervington
Feb 18, 2017·Immunopharmacology and Immunotoxicology·Yasmin Moustafa AhmedAli Ahmed Abo-Saif

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
fluorescence imaging

Software Mentioned

GraphPad
CTAn
GraphPad Prism
CTvol

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.