PMID: 16520653Mar 8, 2006Paper

Effect of methylation-associated silencing of the death-associated protein kinase gene on nasopharyngeal carcinoma

Anti-cancer Drugs
Wei-Jia KongWen Kong

Abstract

Death-associated protein kinase (DAPK) is a Ca/calmodulin-regulated serine/threonine kinase and a positive mediator of apoptosis. Loss of expression of the DAPK gene by aberrant promoter methylation may play an important role in cancer development and progression. The aim of this study was to investigate the frequency of gene promoter methylation of DAPK in nasopharyngeal carcinoma (NPC) and the effect of 5-Aza-2'-deoxycytidine (5-Aza-CdR), a demethylating agent, on CNE cells, a human nasopharyngeal carcinoma cell line, and on xenografts of CNE cells. Methylation-specific PCR and RT-PCR were used to determine the promoter methylation status and mRNA expression of the DAPK gene in NPC. Furthermore, CNE cells were treated in vitro and in vivo with 5-Aza-CdR to explore the effect of demethylating agents on DAPK mRNA expression and tumor growth. Hypermethylation of the DAPK gene promoter was found in 35 (76.1%) of 46 NPC samples. There was no significant difference in the promoter hypermethylation rate among samples from patients with different TNM stages. No promoter hypermethylation of the DAPK gene was found in all six chronic inflammatory nasopharyngeal tissue specimens. DAPK mRNA expression was not detected in NPC tumor specim...Continue Reading

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Citations

Dec 1, 2007·Head and Neck Pathology·Bayardo Perez-Ordoñez
Jan 4, 2007·Molecular Cancer·William Chi-Shing Cho
Dec 7, 2013·Apoptosis : an International Journal on Programmed Cell Death·Yide HuangYao Lin
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Jun 28, 2018·Medicinal Research Reviews·Ahmed Karam Farag, Eun Joo Roh

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