Effect of methylglyoxal on reactive oxygen species, KI-67, and caspase-3 expression in MCF-7 cells

Experimental and Molecular Pathology
Dyah Paramita, J D P Wisnubroto

Abstract

Methylglyoxal (MG) is an extremely reactive α-ketoaldehyde endogenously produced by various metabolic pathways, including the dephosphorylation of glycolytic intermediates, metabolites of the polyol pathway, and aminoacetone metabolism. MG cytotoxicity occurs through the induction of apoptosis. There are multiple mechanisms by which MG can induce apoptosis, e.g., through the generation of reactive oxygen species (ROS), induction of oxidative DNA damage, or accumulation of a specific MG-derived advanced glycation end product (AGE). This study aimed to investigate the effect of MG on ROS production, KI-67 expression, and caspase-3 expression in MCF-7 cells. At confluency, MCF-7 cells were divided into five groups, including control (untreated); and MCF-7 cells exposed to MG (1 mM) for 0; 12; 24; and 48 h of incubation. Reactive oxygen species level, KI-67 expression, and caspase-3 expression were evaluated by laser scanning confocal microscopy. The level of reactive oxygen species was significantly lower in 12 h of incubation compared to the control group (p < .05). The reactive oxygen species level was significantly higher in 48 h of incubation compared to the control group (p < .05). The expressions of KI-67 was significantly h...Continue Reading

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