Feb 1, 1977

Effect of Mg2+ and spermine on the kinetics of Ca2+ transport in rat-liver mitochondria

Journal of Bioenergetics and Biomembranes
K E Akerman


Plots relating the initial rate of mitochondrial Ca2+ transport to the Ca2+ concentration (kinetic plots) have a hyperbolic shape in a Ca2+ concentration range of 2.5-100 muM as measured in sucrose or KCl media. In the presence of Mg2+ or a polyamine spermine, which both are competitive inhibitors of Ca2+ binding to low affinity sites at the membrane surface, the shape of the plots becomes sigmoidal. At higher concentrations of these agents linear kinetic plots are obtained as measured in a sucrose medium. In a KCl medium the sigmoidality of the kinetic plots is enhanced by an increase in the Mg2+ or spermine concentration. It is suggested that Mg2+ and spermine affect the kinetics of Ca2+ transport by interfering with Ca2+ binding to low affinity sites of the membrane surface and that the binding of Ca2+ to these sites is the first step of the mitochondrial Ca2+ transport.

  • References17
  • Citations17


Mentioned in this Paper

Mitochondria, Liver
Dose-Response Relationship, Drug
Saccharum officinale, sucrose, cane sugar, Homeopathic preparation
In Vitro [Publication Type]

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