Effect of Mg2+ on guanine nucleotide sensitivity of ligand binding to serotonin1A receptors from bovine hippocampus

Biochemical and Biophysical Research Communications
Shanti KalipatnapuAmitabha Chattopadhyay

Abstract

The serotonin1A (5-HT1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein coupled receptors (GPCRs). We report here that guanine nucleotide sensitivity of agonist binding to hippocampal 5-HT1A receptors is dependent on the concentration of Mg2+. Our results show that agonist binding to 5-HT1A receptors is relatively insensitive to guanine nucleotides in the absence of Mg2+. In contrast to this, the specific antagonist binding is insensitive to guanine nucleotides, even in the presence of Mg2+. These results point out the requirement of an optimal concentration of Mg2+ which could be used in assays toward determining guanine nucleotide sensitivity of ligand binding to GPCRs such as the 5-HT1A receptor. Our results provide novel insight into the requirement and concentration dependence of Mg2+ in relation to guanine nucleotide sensitivity for the 5-HT1A receptor in particular, and GPCRs in general.

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Jun 5, 2004·Cellular and Molecular Neurobiology·Amitabha ChattopadhyayShanti Kalipatnapu
Jun 19, 2004·Molecular Membrane Biology·Vaishali Javadekar-Subhedar, Amitabha Chattopadhyay
Jun 23, 2004·Cellular and Molecular Neurobiology·Shanti Kalipatnapu, Amitabha Chattopadhyay
Jun 29, 2004·Biochemical and Biophysical Research Communications·Thomas J PucadyilAmitabha Chattopadhyay

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Citations

Aug 22, 2007·Cellular and Molecular Neurobiology·Shanti Kalipatnapu, Amitabha Chattopadhyay
Apr 18, 2006·Progress in Lipid Research·Thomas J Pucadyil, Amitabha Chattopadhyay
Jul 12, 2005·Biochimica Et Biophysica Acta·Thomas J Pucadyil, Amitabha Chattopadhyay
Aug 13, 2005·Molecular Membrane Biology·Yamuna Devi PailaAmitabha Chattopadhyay
Nov 23, 2006·Cellular and Molecular Neurobiology·Yamuna Devi Paila, Amitabha Chattopadhyay

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