Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts

Journal of the Chinese Medical Association : JCMA
Shu-I PaoChang-Min Liang

Abstract

Mesenchymal stem cells (MSCs) are important for regenerative medicine. Limbal fibroblasts (LFs), present in the corneal limbus, have been shown to possess MSC characteristics, and can differentiate into other cell types. The current study sought to investigate the effect of microgravity on the proliferation and differentiation of LFs, and identify culture conditions to obtain a high proportion of LFs possessing MSC characteristics. A rotary cell culture system was used to generate microgravity. Cellular proliferation and MSC marker (CD14, CD45, CD90, CD105, and SSEA4) expression were evaluated by WST-1 test and flow cytometry, respectively. Differentiation of LFs into adipocytes, osteocytes, and chondrocytes was examined. The effects of LF-conditioned medium on limbal stem cell differentiation were assessed. The cellular proliferation rates under microgravity were significantly lower than those under normal gravity (0.44 vs. 0.18 at 24 h, and 0.70 vs. 0.44 at 48 h, both P ≤ 0.004). Higher proportions of cells expressed CD90 (95.33% vs 81.69%), CD105 (95.32% vs 87.96%), and SSEA4 (68.26% vs 26.13%) under microgravity than under normal gravity. The differentiation potential of LFs was more prominent under microgravity. The LF-con...Continue Reading

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Citations

Mar 30, 2018·Stem Cells and Development·Daniela GrimmAlamelu Sundaresan
Aug 20, 2019·Critical Care Medicine·Guan-Yeu ChenPeng-Hui Wang
Sep 25, 2020·Science Translational Medicine·Yonglong GuoJiansu Chen
Aug 27, 2019·Journal of Tissue Engineering·Rodrigo Sn BarretoMaria Angelica Miglino

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