Effect of modulating glutamate signaling on myelinating oligodendrocytes and their development-A study in the zebrafish model.

Journal of Neuroscience Research
Funda TuranBenjamin Odermatt

Abstract

Myelination is crucial for the development and maintenance of axonal integrity, especially fast axonal action potential conduction. There is increasing evidence that glutamate signaling and release through neuronal activity modulates the myelination process. In this study, we examine the effect of manipulating glutamate signaling on myelination of oligodendrocyte (OL) lineage cells and their development in zebrafish (zf). We use the "intensity-based glutamate-sensing fluorescent reporter" (iGluSnFR) in the zf model (both sexes) to address the hypothesis that glutamate is implicated in regulation of myelinating OLs. Our results show that glial iGluSnFR expression significantly reduces OL lineage cell number and the expression of myelin markers in larvae (zfl) and adult brains. The specific glutamate receptor agonist, L-AP4, rescues this iGluSnFR effect by significantly increasing the expression of the myelin-related genes, plp1b and mbpa, and enhances myelination in L-AP4-injected zfl compared to controls. Furthermore, we demonstrate that degrading glutamate using Glutamat-Pyruvate Transaminase (GPT) or the blockade of glutamate reuptake by L-trans-pyrrolidine-2,4-dicarboxylate (PDC) significantly decreases myelin-related genes ...Continue Reading

References

Feb 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B F Trump, I K Berezesky
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Dec 29, 1999·Nature Medicine·D PittC S Raine
Aug 11, 2000·Journal of Neurochemistry·C C MatthewsP S Fishman
Mar 29, 2001·Physiological Reviews·N Baumann, D Pham-Dinh
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wenbin DengFrances E Jensen
Feb 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hui-Hsin TsaiRobert H Miller
Oct 20, 2006·Neuroscience·R Káradóttir, D Attwell
Feb 13, 2007·Nature Neuroscience·Jennifer L ZiskinDwight E Bergles
Feb 13, 2007·Nature Neuroscience·Maria KukleyDirk Dietrich
May 23, 2007·Neurochemistry International·Amanda L Sheldon, Michael B Robinson
Dec 15, 2007·Glia·Carla TaveggiaJames L Salzer
Jan 8, 2008·Nature Neuroscience·Sarah KucenasBruce Appel
Jan 24, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chen Sok LamUwe Strähle
Feb 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Denise A Zannino, Bruce Appel
Dec 19, 2009·Brain Research Reviews·Dwight E BerglesChristian Steinhäuser
Feb 23, 2010·Nature Neuroscience·Ainhoa EtxeberriaVittorio Gallo
Mar 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lindsay M De BiaseDwight E Bergles
Jan 15, 2013·Nature Methods·Jonathan S MarvinLoren L Looger
Jan 7, 2014·PLoS Biology·Iben LundgaardRagnhildur T Káradóttir
Apr 8, 2015·Nature Neuroscience·Jacob H HinesBruce Appel
Apr 9, 2015·Frontiers in Cellular Neuroscience·Aurélia SahelBrahim Nait-Oumesmar
Oct 27, 2015·Brain Research·Marion BarabanDavid A Lyons
Oct 31, 2015·Experimental Neurology·Ileana MicuPeter K Stys
Nov 21, 2015·Nature Reviews. Neuroscience·R Douglas Fields
Oct 30, 2016·Neuropharmacology·Reshmi Tognatta, Robert H Miller
Oct 30, 2016·Neuropharmacology·Sonia SpitzerRagnhildur T Káradóttir
Jul 1, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ainhoa EtxeberriaJonah R Chan
Aug 18, 2017·Neuron·Christopher W Mount, Michelle Monje
Oct 20, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rafael G Almeida, David A Lyons

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

© 2021 Meta ULC. All rights reserved