Abstract
The guanyl nucleotide binding regulatory protein of retinal rod outer segments, called Gt, that couples the photon receptor rhodopsin with the light-activated cGMP phosphodiesterase, can be resolved into two functional components, alpha t and beta gamma t. The effect of monoclonal antibody binding to the alpha t subunit of Gt on subunit association has been investigated in the present study. It was previously shown that this monoclonal antibody, mAb 4A, blocks interactions with rhodopsin and its epitope was located within the region Arg310-Phe350 at the COOH terminus of the alpha t subunit. In this paper, we show that mAb 4A disrupts the Gt complex. Gt migrates in 5-20% linear sucrose density gradients as a monomer, with a sedimentation coefficient of 4.1 +/- 0.07 S, while in the presence of mAb 4A, the alpha t and beta gamma t subunits show sedimentation coefficients of 7.7 +/- 0.2 and 3.7 +/- 0.1 S, respectively. The beta gamma t subunit migrates with the same sedimentation rate as pure beta gamma t. Nonimmune rabbit IgG does not modify the sedimentation behavior of Gt. The Fab fragment of mAb 4A also dissociates the Gt complex, as suggested by the change of the sedimentation rate of alpha t. This effect of mAb 4A on Gt subun...Continue Reading
References
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G L Wheeler, M W Bitensky
May 7, 1976·Analytical Biochemistry·M M Bradford
Aug 15, 1986·Science·A G AmitR J Poljak
Mar 1, 1987·Nature·P M ColmanR G Webster
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S SheriffD R Davies
Aug 12, 1988·Science·A YataniA M Brown
Jan 1, 1987·Annual Review of Physiology·P A LiebmanE A Dratz
Jan 1, 1987·Annual Review of Physiology·J B Hurley
Aug 10, 1987·FEBS Letters·V N Hingorani, Y K Ho
Jan 1, 1986·Annual Review of Cell Biology·L Stryer, H R Bourne
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Jul 14, 1987·Biochemistry·M Wessling-Resnick, G L Johnson
Aug 12, 1988·Science·H E HammK P Hofmann
Oct 16, 1987·Journal of Chromatography·C KleussG Schultz
May 21, 1974·Biochemistry·D S Papermaster, W J Dreyer
Aug 15, 1970·Nature·U K Laemmli
Aug 1, 1984·The Journal of General Physiology·H E Hamm, M D Bownds
Feb 7, 1980·Nature·H Kühn
Aug 1, 1984·The Journal of General Physiology·P L WittM D Bownds
Citations
Jan 1, 1994·Protein Science : a Publication of the Protein Society·E J Neer
Dec 1, 1991·Cellular and Molecular Neurobiology·H E Hamm
Mar 9, 2002·Molecular and Cellular Biology·Alexander PulvermüllerUwe Wolfrum
Apr 1, 1993·Journal of Protein Chemistry·M R Mazzoni, H E Hamm
May 27, 1996·FEBS Letters·M YillaH L Ploegh
Mar 13, 2010·Journal of Chromatography. a·Chao WangHailin Wang
Jun 13, 1997·Cell·G W FarrA L Horwich
Mar 24, 1992·Biochemistry·T MurakamiA M Spiegel
Nov 22, 1996·The Journal of Biological Chemistry·M R Mazzoni, H E Hamm
Jan 25, 1996·Nature·D G LambrightP B Sigler
Jun 15, 2004·Prostate Cancer and Prostatic Diseases·Y LiB J Allen
Jan 8, 2000·The Journal of Biological Chemistry·D S EvankoP B Wedegaertner
Feb 17, 2006·Journal of Medicinal Chemistry·Katharina J GuentherJohn F Valliant
May 21, 1993·Cell·B R Conklin, H R Bourne