Effect of monosaccharides and ethyleneglycol on the interaction between Escherichia coli bacteria and Octyl-Sepharose

Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology
L OhmanO Stendahl


Combined effects of monosaccharides and reduced surface tension of the medium were studied in relation to the hydrophobic binding of Escherichia coli bacteria, with and without mannose-specific structures. Hydrophobic binding was analyzed by hydrophobic interaction chromatography on Octyl-Sepharose. The results showed that ethyleneglycol, as well as mannose, reduced the hydrophobic interaction of the bacteria with mannose-specific structures. This effect was potentiated by combining ethyleneglycol and mannose. No other monosaccharides tested (galactose and fucose) had any effect on the hydrophobic interaction of bacteria with mannose-specific structures. These results further strengthen the hypothesis that the mannose-specific interaction of Escherichia coli bacteria is, at least in part, mediated by hydrophobic forces.


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Jul 1, 1967·Nature·J P Duguid, E S Anderson
Apr 29, 1982·Biochemical and Biophysical Research Communications·N FironN Sharon

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octyl-sepharose CL-4B
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