PMID: 6409998Sep 1, 1983Paper

Effect of monovalent cations on Na+/Ca2+ exchange and ATP-dependent Ca2+ transport in synaptic plasma membranes

Journal of Neurochemistry
O P CoutinhoC M Carvalho

Abstract

Two Ca2+ transport systems were investigated in plasma membrane vesicles isolated from sheep brain cortex synaptosomes by hypotonic lysis and partial purification. Synaptic plasma membrane vesicles loaded with Na+ (Na+i) accumulate Ca2+ in exchange for Na+, provided that a Na+ gradient (in leads to out) is present. Agents that dissipate the Na+ gradient (monensin) prevent the Na+/Ca2+ exchange completely. Ca2+ accumulated by Na+/Ca2+ exchange can be released by A 23187, indicating that Ca2+ is accumulated intravesicularly. In the absence of any Na+ gradient (K+i-loaded vesicles), the membrane vesicles also accumulate Ca2+ owing to ATP hydrolysis. Monovalent cations stimulate Na+/Ca2+ exchange as well as the ATP-dependent Ca2+ uptake activity. Taking the value for Na+/Ca2+ exchange in the presence of choline chloride (external cation) as reference, other monovalent cations in the external media have the following effects: K+ or NH4+ stimulates Na+/Ca2+ exchange; Li+ or Cs+ inhibits Na+/Ca2+ exchange. The ATP-dependent Ca2+ transport system is stimulated by increasing K+ concentrations in the external medium (Km for K+ is 15 mM). Replacing K+ by Na+ in the external medium inhibits the ATP-dependent Ca2+ uptake, and this effect is...Continue Reading

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Citations

Feb 1, 1994·Journal of Biochemical Toxicology·W R MundyH A Tilson
Feb 9, 1994·Molecular and Cellular Biochemistry·R Sandhir, K D Gill
Feb 1, 1990·The Journal of Membrane Biology·L Lagnado, P A McNaughton
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Feb 1, 2005·Basic & Clinical Pharmacology & Toxicology·Amarpreet Kaur, Kiran Dip Gill

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