Effect of mouse VEGF164 on the viability of hydroxyethyl methacrylate-methyl methacrylate-microencapsulated cells in vivo: bioluminescence imaging

Journal of Biomedical Materials Research. Part a
Dangxiao ChengMichael V Sefton

Abstract

Bioluminescent imaging was used to track the viability of luciferase transfected L929 cells in poly(hydroxyethyl methacrylate-co-methyl methacrylate) (HEMA-MMA) microcapsules. Bioluminescence, as determined by Xenogen imaging after addition of luciferin to microcapsules in vitro, increased with time, consistent with an increase in cell number. Capsules were suspended in Matrigel and injected subcutaneously. The bioluminesence in vivo increased over the first 3 weeks and then decreased, both with and without the delivery of mVEGF(164) (1.2 ng/24 h/200 microcapsules in vitro); VEGF delivery was from microencapsulated doubly transfected cells (both luciferase and mVEGF(164)). VEGF delivery was sufficient to generate a greater number of vascular structures, but this did not result in the expected increase in microencapsulated cell viability. Interestingly, the number of vessels at day 28 was less than at day 21, consistent with what would be an expected reduction in VEGF secretion when cell viability is lost. The results presented here do not support the hypothesis that transfection of microencapsulated cells with VEGF is sufficient to correct the oxygen transport limitation, at least with this type of tissue engineering construct....Continue Reading

References

Mar 1, 1977·Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology·F Melsen, L Mosekilde
Jun 9, 1998·Annals of the New York Academy of Sciences·E S Avgoustiniatos, C K Colton
Jun 9, 1998·Annals of the New York Academy of Sciences·M V SeftonJ E Babensee
Jan 19, 1999·Annual Review of Cell and Developmental Biology·T Wilson, J W Hastings
Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I AjiokaY Watanabe
Jul 15, 2000·Journal of Biomaterials Science. Polymer Edition·M Feng, M V Sefton
Sep 23, 2000·Nature·G D YancopoulosJ Holash
Oct 6, 2000·The Journal of Clinical Investigation·Z G ZhangM Chopp
Apr 3, 2001·Nature Biotechnology·R Weissleder
Nov 2, 2001·Nature Biotechnology·P Carmeliet, E M Conway
Nov 2, 2001·Nature Biotechnology·T P RichardsonD J Mooney
Dec 1, 2001·Journal of Biomedical Optics·B W RiceM B Nelson
Apr 12, 2002·Journal of Biomedical Materials Research·Martin C PetersDavid J Mooney
Feb 8, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·David S ChangLouis M Messina
Jun 5, 2003·Nature Medicine·Rakesh K Jain
Jun 19, 2003·The Journal of Clinical Investigation·Yunjuan SunDavid A Greenberg
Oct 31, 2003·Neurochemical Research·Richard L Benton, Scott R Whittemore
Nov 8, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Shayn M PeirceThomas C Skalak
Feb 28, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·Hiroaki YokomoriHiromasa Ishii
Mar 23, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·T D Le CrasA L Akeson
Sep 8, 2005·Tissue Engineering·Paolo De CoppiShay Soker
Dec 15, 2005·Proceedings of the American Thoracic Society·Ruxana T Sadikot, Timothy S Blackwell
Dec 16, 2005·Nature·Peter Carmeliet
Jan 25, 2006·Experimental Cell Research·Harold F Dvorak
Mar 21, 2006·Vascular Pharmacology·Nilesh M PandyaDev D Santani
Feb 20, 2007·The Annals of Thoracic Surgery·Terrence M YauRen-Ke Li
May 26, 2007·Tissue Engineering·Jennifer J Vallbacka, Michael V Sefton

❮ Previous
Next ❯

Citations

Nov 20, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Anna Aihua LiMurray A Potter
Apr 29, 2014·Journal of Biomedical Materials Research. Part a·Ronke M Olabisi
Feb 29, 2020·Biomaterials Science·Barbara Kupikowska-Stobba, Dorota Lewińska
Jul 23, 2014·Journal of Tissue Engineering and Regenerative Medicine·Aubrey R Tiernan, Athanassios Sambanis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.