PMID: 29071907Jun 25, 2016Paper

Effect of Moxibustion and Electroacupuncture on Th 1/Th 2 Immune Balance in Rats with Experimental Colitis

Zhen ci yan jiu = Acupuncture research
Yan-Ping Chen

Abstract

To observe the effect of moxibustion and electroacupuncture (EA) stimulation of "Tianshu" (ST 25) and "Qihai" (CV 6) on Th 1/Th 2 immune balance in rats with experimental ulcerative colitis (UC). The UC rats were randomly allocated to model group, moxibustion group and EA group (n=10 in each group). Another 10 rats were used as the control group. The UC model was established by injection of fresh colonic mucosal tissue-made antigen emulsifier (purified mucosal supernate plus complete Freund's adjuvant, 3.5 mg on day one, and 7 mg thereafter) into the bilateral toes, inguinal grooves, back, and the abdominal cavity on day 1, 10, 17 and 24, respectively. Medicinal paste (containing Cortex Cinnamomi,Flos Carthami, etc.)-separated moxibustion or EA (200 Hz, 2 mA) was applied to ST 25 and CV 6 for 20 min, once daily for 14 days. The mRNA expression levels of interferon-γ (INF-γ), IL-12, IL-4 and IL-10 in colon tissues were detected using quantitative RT-PCR and their content determined using enzyme linked immunosorbent assay (ELISA). The colon tissue cell ratios of CD 4+ INF-γ+ and CD 4+ IL-4+ were analyzed by flow cytometry. Compared with the control group, the mRNA and contents of colonic INF-γ and IL-12 and the ratio of CD 4+ INF...Continue Reading

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