PMID: 26828698Feb 2, 2016Paper

Effect of MTHFR Gene Polymorphism Impact on Atherosclerosis via Genome-Wide Methylation

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Xuefeng LinAiqun Ma

Abstract

Atherosclerosis seriously threats human health. Homocysteine is an independent risk factor closely related to DNA methylation. MTHFR C667T loci polymorphism is closely associated with homocysteine level. This study aimed to investigate the relationship among MTHFR C667T loci polymorphism, genome-wide methylation, and atherosclerosis. Blood sample was collected from 105 patients with coronary atherosclerosis and 105 healthy controls. Pyrosequencing methylation was used to detect LINE-1 methylation level. Polymerase chain reaction-restriction enzyme fragment length polymorphism (PCR-RFLP) was used to test MTHFR. LINE-1 methylation level in the patient group was significantly lower than in the controls (t=5.007, P<0.001). MTHFR C667T genotype distribution presented marked differences in the 2 groups. TT genotype carriers had significantly increased risk of atherosclerosis (OR=3.56, P=0.009). Three different genotypes of MTHFR C667T loci showed different LINE-1 methylation level between the 2 groups (P<0.01). LINE-1 methylation level in TT and CT genotype carriers was obviously lower than in CC genotype carriers (P<0.05). MTHFR C667T loci polymorphism may affect atherosclerosis by regulating genome methylation level.

Citations

May 23, 2020·Current Atherosclerosis Reports·Minghua Liu, Jose Gutierrez
Jan 25, 2017·BioMed Research International·Ha Won Kim, Brian K Stansfield
Jan 20, 2018·Oxidative Medicine and Cellular Longevity·Gang LiuYulong Yin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.