PMID: 7019625Jan 1, 1981Paper

Effect of muramyldipeptide, a synthetic bacterial adjuvant, on enzyme release from cultured mouse macrophages

Microbiology and Immunology
K Imai, A Tanaka

Abstract

Monolayer cultures of macrophages obtained by peritoneal lavage of normal or thioglycollate-stimulated mice spontaneously secreted lysosomal enzymes into the culture medium. When the elicited macrophages were cultured in the presence of muramyldipeptide (MDP), a 20-30% increase in the release of beta-glucuronidase was consistently observed and the intracellular activity decreased to about 45% of that of control cells after 6-8 days' culture. A stimulatory effect of MDP on lysozyme secretion, though less profound, was also observed. In contrast, release of neither enzyme was stimulated in resident macrophages by the addition of MDP. A neutral alpha-glucosidase, which has recently been found to localize also in granules of macrophages, remained inside the cells and neither its activity nor its release was affected by the addition of MDP to either type of macrophages. A large amount of lactic dehydrogenase was released only when the resident, not the elicited, macrophages were cultured for 3-4 days and then phagocytosed zymosan.

References

Aug 1, 1978·The Journal of Experimental Medicine·J Schnyder, M Baggiolini
Feb 1, 1978·Infection and Immunity·K Emori, A Tanaka
Aug 1, 1978·European Journal of Immunology·M FevrierP Liacopoulos
May 1, 1979·Infection and Immunity·A TanakaS Kusumoto
May 1, 1979·Infection and Immunity·S NagaoS Kotani
Jul 1, 1979·Infection and Immunity·H TakadaK Yokogawa
Feb 28, 1978·Biochemical and Biophysical Research Communications·Y YamamotoK Onoue
Jan 1, 1977·Infection and Immunity·A TanakaS Kotani
Jul 25, 1977·Biochemical and Biophysical Research Communications·A TanakaT Shiba
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R M Pantalone, R C Page
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L ChedidE Lederer
Feb 1, 1973·The Journal of Experimental Medicine·C F NathanJ R David
Jan 1, 1973·The Journal of Infectious Diseases·K Brune, J K Spitznagel
Aug 19, 1974·Biochemical and Biophysical Research Communications·F EllouzE Lederer
May 1, 1974·The Journal of Experimental Medicine·S GordonZ A Cohn
Jan 1, 1969·The Journal of Cell Biology·R H MichellM L Karnovsky
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton
Jul 1, 1964·The Journal of Experimental Medicine·G B MACKANESS

❮ Previous
Next ❯

Citations

Jan 1, 1992·Microbiology and Immunology·S NagaoY Tanigawa
Oct 1, 1988·Cell Biochemistry and Function·Y TakanoK Yamamoto
Apr 1, 1984·Medicinal Research Reviews·A Adam, E Lederer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.