Abstract
Defensins are small cationic peptides that constitute the first line of defense against pathogens and are involved in immune regulation. In this study, their role in peripheral nerve regeneration was investigated. Rat sciatic nerves were transected and the two nerve stumps were bridged by a chitin conduit with a gap of 5mm between the stumps. The animals were injected intramuscularly with mutated rabbit neutrophil peptide 1 (defensin mNP-1), the positive control nerve growth factor (NGF) or the negative control saline, for 7 consecutive days after repair. After 6 weeks, the sciatic functional index (SFI), MNCV (motor nerve conductive velocity) and morphological parameters including myelinated fiber amounts, fiber diameter, axon diameter, myelin thickness and G-ratio were measured. Compared to the SFI of saline group, the NGF and mNP-1 groups had an increase of 18.3% and 18.8%, respectively. The numbers of myelinated fibers in the distal nerve of NGF and mNP-1 groups were 1.45- and 1.32-fold higher than in the saline group. The MNCVs of NGF and mNP-1 groups were 7.3 and 4.4 times of that of saline group. Fiber diameter, axon diameter, myelin thickness and G-ratio in the NGF and mNP-1 groups were also significantly higher than th...Continue Reading
References
Dec 1, 1989·The Journal of Clinical Investigation·M C TerritoR Lehrer
Oct 1, 1985·The Journal of Clinical Investigation·T GanzR I Lehrer
Jan 1, 1987·Experimental Brain Research·R B BhisitkulJ D Kocsis
Sep 1, 1995·Journal of Neurocytology·C Fernandez-ValleM B Bunge
Oct 1, 1994·Neuron·L McKerracherP E Braun
Feb 15, 1993·FEBS Letters·D VerbanacD Romeo
Nov 1, 1996·Immunopharmacology·M IchinoseM Sawada
Jun 16, 2000·Neuroscience·V CoronasR Quirion
Nov 10, 2001·Cell and Tissue Research·J R GarcíaR Bals
May 16, 2002·Experimental Neurology·Olawale A R SulaimanTessa Gordon
Jun 20, 2002·Trends in Immunology·De YangJoost J Oppenheim
Aug 3, 2002·Immunology·Louise A DuitsPeter H Nibbering
Sep 6, 2003·Experimental Neurology·Esther UdinaXavier Navarro
Sep 23, 2003·Journal of Molecular Neuroscience : MN·Shlomo Rotshenker
Apr 1, 2003·Integrative and Comparative Biology·Tomas Ganz
Feb 6, 2013·PloS One·Li-Li BaiZan-Min Hu
Citations
Sep 6, 2019·Scientific Reports·Chengming FanZanmin Hu
May 13, 2020·Neural Regeneration Research·Yu-Song YuanYu-Hui Kou
Oct 16, 2020·Nutritional Neuroscience·Fei YuBaoguo Jiang