PMID: 9539704May 16, 1998Paper

Effect of mutating the regulatory phosphoserine and conserved threonine on the activity of the expressed catalytic domain of Acanthamoeba myosin I heavy chain kinase

Proceedings of the National Academy of Sciences of the United States of America
J SzczepanowskaH Brzeska

Abstract

Phosphorylation of Ser-627 is both necessary and sufficient for full activity of the expressed 35-kDa catalytic domain of myosin I heavy chain kinase (MIHCK). Ser-627 lies in the variable loop between highly conserved residues DFG and APE at a position at which a phosphorylated Ser/Thr also occurs in many other Ser/Thr protein kinases. The variable loop of MIHCK contains two other hydroxyamino acids: Thr-631, which is conserved in almost all Ser/Thr kinases, and Thr-632, which is not conserved. We determined the effects on the kinase activity of the expressed catalytic domain of mutating Ser-627, Thr-631, and Thr-632 individually to Ala, Asp, and Glu. The S627A mutant was substantially less active than wild type (wt), with a lower kcat and higher Km for both peptide substrate and ATP, but was more active than unphosphorylated wt. The S627D and S627E mutants were also less active than phosphorylated wt, i.e., acidic amino acids cannot substitute for phospho-Ser-627. The activity of the T631A mutant was as low as that of the S627A mutant, whereas the T632A mutant was as active as phosphorylated wt, indicating that highly conserved Thr-631, although not phosphorylated, is essential for catalytic activity. Asp and Glu substitutions...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·S S TaylorJ M Sowadski
Nov 17, 1995·The Journal of Biological Chemistry·Z Y WangE D Korn
Feb 1, 1994·Protein Science : a Publication of the Protein Society· MadhusudanJ M Sowadski
May 15, 1994·Structure·S S Taylor, E Radzio-Andzelm
Feb 24, 1994·Nature·F ZhangE J Goldsmith
Oct 1, 1993·Protein Science : a Publication of the Protein Society·J ZhengL F Ten Eyck
Jul 19, 1996·The Journal of Biological Chemistry·H Brzeska, E D Korn
Aug 1, 1996·Nature Structural Biology·A A RussoN P Pavletich
Dec 13, 1996·The Journal of Biological Chemistry·C WuE Leberer
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·H BrzeskaE D Korn
Apr 1, 1997·Trends in Cell Biology·M A Sells, J Chernoff

❮ Previous
Next ❯

Citations

Nov 19, 2002·International Journal for Parasitology·Christian DoerigDebopam Chakrabarti
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·H BrzeskaE D Korn
Jul 22, 2015·Current Opinion in Chemical Biology·Zan Chen, Philip A Cole
Apr 12, 2006·Cell Motility and the Cytoskeleton·Joanna SzczepanowskaHanna Brzeska
Aug 31, 2007·The Journal of Biological Chemistry·Christopher P MattisonMark Winey
Apr 24, 2020·Organic & Biomolecular Chemistry·Mamidi SamarasimhareddyAssaf Friedler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.