Effect of mutations in Pisum sativum L. genes blocking different stages of nodule development on the expression of late symbiotic genes in Rhizobium leguminosarum bv. viciae

Molecular Plant-microbe Interactions : MPMI
Vera A VoroshilovaU B Priefer

Abstract

In this report, the expression of late symbiotic genes (fnrN, fixN, and nifA) of Rhizobium leguminosarum bv. viciae was studied in nodules of mutant pea lines blocked at four successive stages of nodule development. Bacterial gene expression was analyzed in situ with transcriptional gusA reporter gene fusions. As a control, a constitutively expressed gusA gene was included. In the nodules of Nop(nodule persistence) mutants (mutant in gene sym13), which had not yet exhibited signs of premature senescence, the expression patterns observed were identical to those in wild-type nodules. Normal expression of fusions also occurred in nodules defective at the infection droplet differentiation stage (mutant in gene sym40) in which bacteria are endocytosed, but infection threads and infection droplets are hypertrophied. In contrast, in Itn- (infection thread formation inside the nodule tissue) mutants (mutant gene sym33), in which there is no endocytosis of bacteria, expression of the constitutive fusion was only in infection threads and no activity was shown for the other fusions. From this it can be concluded that functionality of the plant gene Sym33, i.e., bacterial endocytosis, is a prerequisite for the expression of late symbiotic ...Continue Reading

References

Jun 25, 1992·Nature·R F Fisher, S R Long
Jan 1, 1991·Annual Review of Microbiology·G Caetano-Anollés, P M Gresshoff
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·E SoupèneJ Batut
Jul 1, 1997·Molecular Plant-microbe Interactions : MPMI·A SchlüterU B Priefer
Oct 28, 1998·Molecular & General Genetics : MGG·V E TsyganovI A Tikhonovich
Feb 3, 1999·Annual Review of Genetics·M Schultze, A Kondorosi
Apr 25, 2000·Plant Science : an International Journal of Experimental Plant Biology·E V MorzhinaI A Tikhonovich
Nov 1, 1990·Plant Physiology·B E KneenN F Weeden
Oct 1, 1987·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K C Engvild
Mar 1, 1996·World Journal of Microbiology & Biotechnology·M Schultze, A Kondorosi

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Citations

Jul 17, 2007·Nature Reviews. Microbiology·Kathryn M JonesGraham C Walker
Jul 27, 2011·Molecular Plant-microbe Interactions : MPMI·Evgenia OvchinnikovaErik Limpens
Jul 1, 2005·Molecular Plant-microbe Interactions : MPMI·Lydia J BrightJeanne M Harris
Nov 7, 2008·Molecular Plant-microbe Interactions : MPMI·Vladimir ZhukovJens Stougaard
Apr 10, 2003·Molecular Genetics and Genomics : MGG·N SuganumaM Kawaguchi
Dec 9, 2008·Journal of Bacteriology·Feng-Juan YangChristian Staehelin
Apr 2, 2019·Frontiers in Plant Science·Tatiana A SerovaViktor E Tsyganov
Dec 16, 2020·Plants·Viktor E Tsyganov, Anna V Tsyganova

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