PMID: 11343797May 10, 2001Paper

Effect of N-domain on the stability of elongation factor Ts from Thermus thermophilus

Biochimica Et Biophysica Acta
E ValusováM Sprinzl

Abstract

Elongation factor Ts (EF-Ts) from Thermus thermophilus forms a stable, functionally active homodimer in solution. Its monomer is composed of two domains: amino-terminal domain containing 50 amino acid residues and a larger, 146 residues long, C-domain which participates in dimerization of EF-Ts. Effect of removal of the N-domain on the conformational stability of EF-Ts has been studied. For comparison, the stabilities of both the full-length EF-Ts and its C-domain were studied by differential scanning calorimetry, electronic absorption and fluorescence spectroscopies over a pH range from 4 to approximately 13. Thermal denaturation of EF-Ts and of C-domain, followed by circular dichroism at 222 nm, at pH 7.0, and the pH dependence of the fluorescence of the single tryptophan 30 residue indicate a conformational instability of the N-domain. While N-domain does not affect the stability of full-length EF-Ts at acidic pH, its removal leads to stabilization of the rest of the protein at basic pH. This is reflected by higher values of transition temperatures and calorimetric enthalpies of C-domain as compared to the full-length EF-Ts. High mobility of the N-domain in alkaline pH conditions decreased the thermal stability of covalently...Continue Reading

References

Jan 1, 1979·Advances in Protein Chemistry·P L Privalov
Apr 1, 1990·Trends in Biotechnology·C N Pace
Oct 1, 1989·Protein Engineering·A V Finkelstein, J Janin
Jan 13, 1987·Biochemistry·C Q Hu, J M Sturtevant
Nov 1, 1987·Biopolymers·W J Becktel, J A Schellman
Aug 5, 1986·Journal of Molecular Biology·P L PrivalovV P Kutyshenko
Nov 1, 1994·Protein Science : a Publication of the Protein Society·G I Makhatadze, M A Marahiel
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Aug 1, 1997·Nature Structural Biology·Y WangP B Sigler
Jan 7, 1998·Journal of Molecular Biology·A Tamura, P L Privalov
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·V VilleretJ Van Beeumen
May 30, 1998·Protein Science : a Publication of the Protein Society·D L Allen, G J Pielak
Mar 3, 1999·American Journal of Human Genetics·J M SilvermanK L Davis
May 11, 1999·Protein Engineering·M Karplus, J Janin

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