Abstract
To determine the effect on BCG of n-octanesulphonylacetamide (OSA), a novel compound of the class beta-sulphonylcarboxamides, which has potent in vitro activity against pathogenic mycobacteria. The effect of OSA in BCG was examined using two-dimensional protein electrophoresis. Treatment of BCG with OSA resulted in overexpression of two proteins identified as the b-subunit of ATP synthase (Rv1306) and a 17 kDa heat shock protein (Rv0251c). [35S]Methionine pulse-labelling revealed that overexpression occurred within as little as 3.5 h post-exposure. These results were confirmed by RT-PCR. ATP levels decreased in OSA-treated BCG at 5 min, and 1, 3 and 24 h, with a 64%, 45%, 54% and 73% reduction in ATP, respectively. Only dicyclohexylcarbodiimide (DCCD), a known ATP synthase inhibitor, had a similar effect. No appreciable difference in ATP level was observed in BCG treated with standard antimycobacterial drugs, additional respiratory chain inhibitors or a fatty acid synthase inhibitor at a comparable time-point. Protein synthesis decreased within 5 min of exposure to OSA (56%), DCCD (74%) and thenoyltrifluoroacetone (TTFA) (77%). Ethanol (2.3%) potentiated the activity of OSA. In contrast, no synergic effect was observed with str...Continue Reading
References
Aug 11, 1992·Annals of Internal Medicine·S W DooleyD E Snider
May 1, 1992·The Journal of Antimicrobial Chemotherapy·D A Mitchison
Dec 1, 1985·Journal of General Microbiology·Y N Lee, M J Colston
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·B JIJ Grosset
Mar 9, 1994·JAMA : the Journal of the American Medical Association
Feb 25, 1993·The New England Journal of Medicine·T R FriedenS W Dooley
Aug 22, 1997·The Journal of Biological Chemistry·D T McLachlin, S D Dunn
Mar 17, 1998·Biochemical and Biophysical Research Communications·A StruglicsJ F Allen
Jun 11, 1998·Science·K MdluliC E Barry
Jul 3, 1998·FEBS Letters·T L CavistonB D Cain
Dec 31, 1998·Antimicrobial Agents and Chemotherapy·E MiyazakiW R Bishai
Feb 20, 1999·Cell·U JakobJ C Bardwell
May 1, 1999·Antimicrobial Agents and Chemotherapy·M H CynamonS Chase
May 21, 1999·The Journal of Biological Chemistry·O DmitrievR H Fillingame
Oct 16, 1999·The Journal of Biological Chemistry·M RevingtonS D Dunn
Apr 5, 2000·The Journal of Antimicrobial Chemotherapy·J Fung-TomcD Bonner
Jun 6, 2000·Biochimica Et Biophysica Acta·S D DunnM Revington
Jul 6, 2000·Nature·C K StoverW R Baker
Jul 7, 2000·The Journal of Biological Chemistry·P C JonesR H Fillingame
Aug 15, 2000·Gene·F MarianiV Colizzi
Sep 1, 2000·Journal of Medicinal Chemistry·P B JonesC A Townsend
Sep 1, 2000·The European Respiratory Journal·V SchwoebelH L Rieder
Feb 22, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W M El-SadrD L Cohn
Mar 17, 2001·The Journal of Antimicrobial Chemotherapy·N M ParrishJ D Dick
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·N M ParrishJ D Dick
Aug 8, 2001·Molecular Microbiology·R ManganelliI Smith
May 3, 2002·Biochimica Et Biophysica Acta·Marie-Christine BeauvieuxJean-Louis Gallis
Sep 11, 2002·Biochimica Et Biophysica Acta·Sharon H Ackerman
Oct 9, 2002·Microbiology·Graham R StewartPhilip D Butcher
Citations
Jul 12, 2011·American Journal of Therapeutics·Sujata Sharma, Mark A Yoder
May 15, 2012·European Journal of Medicinal Chemistry·M M ShulaevaV S Reznik
Aug 16, 2016·European Journal of Medicinal Chemistry·Jun-Jie KohShouping Liu
Mar 13, 2014·Antimicrobial Agents and Chemotherapy·Philippa A BlackBavesh D Kana
Feb 19, 2015·MBio·Željka MaglicaJohn D McKinney
May 1, 2008·Expert Review of Vaccines·Lynn G DoverGurdyal S Besra
May 9, 2012·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Philip G CrandallNicole M Parrish
Oct 29, 2008·The Journal of Biological Chemistry·William W MohnLindsay D Eltis
Feb 15, 2017·Frontiers in Microbiology·Amar M YewareDhiman Sarkar
Oct 30, 2020·Microbiology and Molecular Biology Reviews : MMBR·Alexander Cambré, Abram Aertsen
Feb 25, 2017·Microbiology Spectrum·Ben Gold, Carl Nathan
Feb 13, 2007·Bioorganic & Medicinal Chemistry·Yves L Janin