Effect of N-terminal truncation and solution conditions on chemokine dimer stability: nuclear magnetic resonance structural analysis of macrophage inflammatory protein 1 beta mutants

Biochemistry
J S LaurenceP J LiWang

Abstract

Chemokines (chemotactic cytokines) are a family of immune system proteins, several of which have been shown to block human immunodeficiency virus (HIV) infection in various cell types. While the solved structures of most chemokines reveal protein dimers, evidence has accumulated for the biological activity of individual chemokine monomers, and a debate has arisen regarding the biological role of the chemokine dimer. Concurrent with this debate, several N-terminal truncations and modifications in the CC subfamily of chemokines have been shown to have functional significance, in many cases antagonizing their respective receptors and in some cases retaining the ability to block HIV entry to the cell. As the dimer interface of CC chemokines is located at their N-terminus, a structural study of N-terminally truncated chemokines will address the effect that this type of mutation has on the dimer-monomer equilibrium. We have studied the structural consequences of N-terminal truncation in macrophage inflammatory protein 1 beta (MIP-1 beta), a CC chemokine that has been shown to block HIV infection. Examination of nuclear magnetic resonance (NMR) spectra of a series of N-terminally truncated MIP-1 beta variants reveals that these protei...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
May 1, 1995·Journal of Leukocyte Biology·I Clark-LewisB D Sykes
Sep 23, 1994·Journal of Molecular Biology·W J FairbrotherR Horuk
Apr 1, 1994·Science·K RajarathnamI Clark-Lewis
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Sep 1, 1995·Journal of Biomolecular NMR·D S WishartB D Sykes
Oct 3, 1996·Nature·F Arenzana-SeisdedosM Baggiolini
Apr 11, 1997·The Journal of Biological Chemistry·W Koopmann, M S Krangel
Nov 14, 1997·Biochemistry·A J HoogewerfT N Wells

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Citations

Feb 19, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·B ComuzziR Paroni
Nov 5, 1999·Protein Science : a Publication of the Protein Society·M P CrumpB D Sykes
Dec 14, 1999·Protein Science : a Publication of the Protein Society·A C LiwangP J Liwang
May 27, 2006·Journal of Molecular Biology·Christopher T VeldkampBrian F Volkman
Nov 1, 2005·Biochemical and Biophysical Research Communications·Hongjun JinPatricia J LiWang
Jul 21, 2017·International Journal of Molecular Sciences·Anna F NguyenPatricia J LiWang
Jul 24, 2007·The Journal of Biological Chemistry·Hongjun JinPatricia J LiWang
Mar 13, 2002·The Journal of Biological Chemistry·E Sonay KuloğluBrian F Volkman
Jun 19, 2002·The Journal of Biological Chemistry·Ennan GuanMichael A Norcross

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