PMID: 29072008Oct 27, 2017Paper

Effect of "Nape Seven Needles" on Expression of Dll 4 and Hes 1 in Cervical Intervertebral Disc Degeneration Rats

Zhen ci yan jiu = Acupuncture research
Yan LuYong-Chen Zhang

Abstract

To observe the effect of "Nape Seven Needles" on the expressions of Delta-like ligand 4 (Dll 4) and hairy and enhancer of split homolog 1(Hes 1) in cervical intervertebral disc degeneration(IVDD) rats, so as to explore its mechanism of deferring the degeneration of cervical intervertebral disc. SD male rats were randomly divided into a normal group, a model group and a nape seven needles group (n=11 in each group). The IVDD model was established by static-dynamic imbalance method. "Nape Seven Needles" acupoints were bilateral "Fengchi" (GB 20), "Tianzhu" (BL 10), "Wangu" (GB 12) and "Fengfu" (GV 16). The rats in the nape seven needles group were treated for 4 weeks, once a day, with needles retained for 20 minutes. Tilted plane test was used to observe the angle changes. The morphology of cervical intervertebral disc was observed after H.E. stain. Dll 4 and Hes 1 mRNA and protein expression in intervertebral disc tissues were detected by real-time fluorescent quantitative PCR and Western blot, respectively. Compared with that in the normal group, the angle of tilted plane in the model group decreased (P<0.05); compared with that in the model group, the angle in the nape seven needles group increased (P<0.05). The structure of c...Continue Reading

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