Effect of neonatal hypothyroidism on the kinetic properties of Na+, K+ -ATPase from rat brain microsomes

Journal of Neuroendocrinology
S S KatyareB N Dave

Abstract

The effects of neonatal hypothyroidism on the kinetic properties of Na+, K+ -ATPase from rat brain microsomes were examined. Neonatal hypothyroidism resulted in decreased Na+, K+ -ATPase activity compared to control samples (7.4 +/- 1.48 and 29.8 +/- 2.30 micromol Pi/h/mg protein, respectively, P < 0.001). Substrate kinetics studies with ATP, Na+ and K+ revealed that there were generalised decreases in Vmax. For ATP, Na+ and K+, activities resolved into two kinetic components in the control group. In hypothyroid animals, the low-affinity component for ATP was absent. The opposite pattern (i.e. an absence of the high-affinity component) was noted for Na+. For K+, although both kinetic components were discernible in neonatal hypothyroid brain microsomes, the Km of the high-affinity component was significantly higher (P < 0.001) compared to control samples. In the control group, the enzyme displayed allosteric behaviour at high concentrations of Mg2+; in hypothyroid animals, the pattern was completely allosteric. The Na+, K+ -ATPase enzyme from the hypothyroid brain microsomes bound two molecules of ATP rather than one, unlike in the control animals. Our results thus indicate that neonatal hypothyroidism results in an impairment o...Continue Reading

References

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Citations

Mar 19, 2014·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Christos KoromilasStylianos Tsakiris
Aug 16, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Christos KoromilasStylianos Tsakiris
Nov 28, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R G Ahmed, S Incerpi
Jul 6, 2010·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·O M AhmedR G Ahmed
Nov 23, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Osama M AhmedR G Ahmed

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