Effect of neural stem cell transplantation combined with erythropoietin injection on axon regeneration in adult rats with transected spinal cord injury

Genetics and Molecular Research : GMR
Y ZhaoY L Xiao

Abstract

We investigated the effect of neural stem cells (NSC) and erythropoietin (EPO) on axon regeneration in adult rats with transected spinal cord injury, and provided an experimental basis for clinical treatment. Forty Wistar rats with T10-transected spinal cord injury were randomly divided into four groups of ten rats: a control group (group A), an NSC-transplant group (group B), an NSC-transplant and EPO group (group C), and an EPO group (group D). Biotinylated dextran amines (BDA) anterograde corticospinal cord neuronal tracing and Fluoro-Gold (FG) retrograde tracing were carried out at the 8th week after operation to observe the regeneration of nerve fibers. The Basso, Beattie, and Bresnahan (BBB) locomotor score was used to evaluate restoration. 1) BDA and FG immunofluorescence staining: in group C, a large number of regenerated axons were observed and some penetrated the injured area. In group B, only a small number of regenerated axons were observed and none penetrated the injured area. In group D, only sporadic regenerated nerve fibers were observed occasionally, while in group A, no axonal regeneration was observed. In group C, a small number of cones and axons emitted yellow fluorescence, and no FG-labeled cells were obse...Continue Reading

Citations

Feb 19, 2017·Neuroscience Letters·Rustem Robertovich IslamovIgor Aleksandrovich Lavrov
Oct 13, 2017·Cell Death & Disease·Yuewen TangLin Cheng
Jun 21, 2017·Stem Cells International·Jing Qu, Huanxiang Zhang
Sep 8, 2021·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Mahdi GholamiAhmad Asghari

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