PMID: 15223828Jun 30, 2004Paper

Effect of NF-kappa B inhibition on TNF-alpha-induced apoptosis in human RPE cells

Investigative Ophthalmology & Visual Science
Ping YangGlenn J Jaffe

Abstract

In many cell types, tumor necrosis factor (TNF)-alpha-induced apoptosis is prevented by production of TNF-alpha-induced antiapoptotic protein, a process mediated by nuclear transcription factor (NF)-kappa B. TNF-alpha is widely expressed in proliferative vitreoretinopathy (PVR) membranes and is present in the vitreous of eyes with PVR. To understand mechanisms responsible for RPE cell survival and death in this disease, this study was conducted to determine whether specific NF-kappa B blockade by mutant inhibitory (I)-kappa B (I kappa B) affects TNF-alpha-induced cell death. Cultured human RPE cells and T-98G cells were infected with adenovirus encoding either beta-galactosidase or mutant I kappa B and then treated with TNF-alpha or interleukin (IL)-1 beta. I kappa B, inhibitor of apoptosis protein (IAP)-1, and cellular Fas-associated death domain-like interleukin-1 beta-converting enzyme-like inhibitory protein (c-FLIP) expression was determined by Western blot. Functional NF-kappa B activation was examined by luciferase reporter assay. Cell viability was evaluated by trypan blue exclusion assay. Caspase-3 activity and DNA fragmentation was measured with an enzyme-linked immunosorbent assay. Mutant I kappa B expression blocked...Continue Reading

Citations

Nov 5, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Panagiotis G TheodossiadisIoannis A Vergados
Apr 14, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cong PengZigang Dong
Dec 10, 2009·Retina·Monica GigantiNaomi Falk
Dec 19, 2013·PloS One·Salvador Pastor-IdoateUNKNOWN Genetics on PVR Study Group
Feb 14, 2014·TheScientificWorldJournal·I PinillaF Sopeña
Feb 15, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yunhe XuAndrew D Dick
Dec 17, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Graça Miguel
Sep 9, 2020·Experimental Eye Research·Alexa KlettnerJohann Roider
Jun 15, 2007·Biochemical and Biophysical Research Communications·Jing MiBryan M Clary

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