Effect of NHE1 antisense gene transfection on the biological behavior of SGC-7901 human gastric carcinoma cells.

World Journal of Gastroenterology : WJG
Hai-Feng LiuXing-Wei Wang

Abstract

To study the effect of type 1 Na(+)/H(+) exchanger (NHE1) antisense human gene transfection on the biological behavior of gastric carcinoma cell line SGC-7901. Antisense NHE1 eukaryotic expression on vector pcDNA3.1 was constructed by recombinant DNA technique and transfected into gastric carcinoma cell line SGC-7901 with DOTAP liposome transfection method. Morphological changes of cells were observed with optic and electron microscopes. Changes in cell proliferative capacity, apoptosis, intracellular pH (pH(i)), cell cycle, clone formation in two-layer soft agar, and tumorigenicity in nude mice were examined. Antisense eukaryotic expressing vectors were successfully constructed and transfected into SGC-7901. The transfectant obtained named 7901-antisense (7901-AS) stablely produced antisense NHE1. There was a significant difference between the pH(i) of 7901-AS cells (6.77 +/- 0.05) and that of 7901-zeo cells and SGC-7901 cells (7.24 +/- 0.03 and 7.26 +/- 0.03, P < 0.01). Compared with SGC-7901 and 7901-zeo cells, 7901-AS cells mostly showed cell proliferation inhibition, G1/G0 phase arrest, increased cell apoptotic rate, recovery of contact inhibition, and density contact. The tumorigenicity in nude mice and cloning efficiency...Continue Reading

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Citations

Sep 29, 2011·Biochemical Genetics·Zhang-yong NingMing Liao
Jan 26, 2011·Journal of Gastroenterology and Hepatology·Xuekang YangKefeng Dou
Feb 9, 2010·Biochemical and Biophysical Research Communications·Ulrike OlszewskiGerhard Hamilton
Apr 23, 2013·Critical Reviews in Biochemistry and Molecular Biology·Alice V Schofield, Ora Bernard
Sep 11, 2014·World Journal of Gastroenterology : WJG·Atsushi ShiozakiYoshinori Marunaka
Feb 6, 2020·Frontiers in Physiology·Li CaoChristian Stock
Mar 30, 2021·Frontiers in Cell and Developmental Biology·Atsushi ShiozakiEigo Otsuji
Nov 16, 2021·Cellular and Molecular Life Sciences : CMLS·Zilin DengXuemei Liu

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