Effect of nicotine on fibroblast beta 1 integrin expression and distribution in vitro

Journal of Periodontology
G W AustinM A Billman

Abstract

Integrins are a family of transmembrane cell surface glycoproteins, and those with the beta 1-subunit function in both cell-to-cell and cell-to-substrate adhesion. The purpose of this study was to determine nicotine's effect on the expression and distribution of the beta 1 integrin subunit on the human gingival fibroblast cell surface. Pure nicotine was diluted in medium to the following concentrations: 0 (control), 0.025, 0.05, 0.1, 0.2, and 0.4 microM. Human gingival fibroblasts (HFG) were grown for 24 hours in each concentration and fluorescein-labeled with a mouse monoclonal anti-human beta 1 antibody and secondarily incubated with a urease-labeled anti-mouse IgG antibody. After a final wash, the cells were incubated with urea/bromcresol blue substrate for 15 minutes at 37 degrees C and measured in a microplate reader at 570 nm. The integrin beta 1-subunit was detected on the HGF surface membrane by fluorescence labeling, and cell-enzyme-linked immunosorbent assay testing demonstrated its decreased expression with increasing nicotine concentrations that were statistically different at the concentrations of 0.2 and 0.4 microM versus controls (P < 0.05). Nicotine concentrations of 0.2 and 0.4 microM significantly decrease bet...Continue Reading

References

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Citations

Aug 23, 2002·European Journal of Pharmacology·Vivian Y ShinChi H Cho
May 25, 2002·Journal of Periodontology·Harold B SnyderGeorge Schuster
Apr 8, 2004·Journal of Periodontology·Georgia K Johnson, Margaret Hill
Oct 6, 2010·Journal of Indian Society of Periodontology·Ranjan MalhotraSumit Kaushal
Apr 25, 2007·Journal of Periodontal Research·J ZhouL J Windsor
Dec 9, 2016·Medicine·Marco TatulloMassimo Marrelli
Nov 1, 2018·Brazilian Oral Research·Rahşan Atasoy ŞentürkBinnaz Handan Özdemir
Dec 11, 2019·Toxics·Gozde Isik AndrikopoulosKonstantinos Poulas

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