Effect of nitrate injection on the bacterial community in a water-oil tank system analyzed by PCR-DGGE

Journal of Industrial Microbiology & Biotechnology
Diogo JureleviciusLucy Seldin

Abstract

Sulfide production by sulfate-reducing bacteria (SRB) is a major concern for the petroleum industry since it is toxic and corrosive, and causes plugging due to the formation of insoluble iron sulfides (reservoir souring). In this study, PCR followed by denaturing gradient gel electrophoresis (PCR-DGGE) using two sets of primers based on the 16S rRNA gene and on the aps gene (adenosine-5-phosphosulfate reductase) was used to track changes in the total bacterial and SRB communities, respectively, present in the water-oil tank system on an offshore platform in Brazil in which nitrate treatment was applied for 2 months (15 nitrate injections). PCR-DGGE analysis of the total bacterial community showed the existence of a dominant population in the water-oil tank, and that the appearance and/or the increase of intensity of some bands in the gels were not permanently affected by the introduction of nitrate. On the other hand, the SRB community was stimulated following nitrate treatment. Moreover, sulfide production did not exceed the permissible exposure limit in the water-oil separation tank studied treated with nitrate. Therefore, controlling sulfide production by treating the produced water tank with nitrate could reduce the quantit...Continue Reading

References

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Citations

Aug 23, 2011·Applied Microbiology and Biotechnology·Lisa M GiegJulia M Foght
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Joana Montezano MarquesElisa Korenblum
Nov 27, 2014·Environmental Science & Technology·Genevieve A KahrilasThomas Borch
Sep 9, 2009·Journal of Hazardous Materials·Yang-Guo ZhaoNan-Qi Ren
Jul 17, 2014·The Journal of Physiology·Nils Hansson, Serge Daan

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