PMID: 8956328Nov 1, 1996Paper

Effect of non-ionic surfactants on the formation of DNA/emulsion complexes and emulsion-mediated gene transfer

Pharmaceutical Research
F LiuD Liu

Abstract

To study the structure-function relationship of non-ionic surfactants in emulsion-mediated gene delivery. Four different types of non-ionic surfactants including Tween, Span, Brij and pluronic copolymers were used as co-emulsifiers for preparation of emulsions composed of Castor oil, dioleoylphosphatidylethanolamine (DOPE) and 3 beta [N-(N', N'-dimethylaminoethane) carbamoyl] cholesterol (DC-Chol). The effect of different surfactants on the formation of DNA/emulsion complexes and transfection activity were analyzed using plasmid DNA containing luciferase cDNA as a reporter gene. Non-ionic surfactants containing branched polyoxyethylene chains as the hydrophilic head group were more effective in preventing the formation of large DNA/emulsion complexes than those containing one or no polyoxyethylene chain. All emulsion formulations except those containing Brij 700 exhibited high activity in transfecting mouse BL-6 cells in the absence of serum. In the presence of serum, however, transfection activity of each formulation varied significantly. Emulsions containing Tween, Brij 72, pluronic F68 and F127 demonstrated increased activity in transfecting cells in the presence of 20% serum. In contrast to emulsions containing Span, long c...Continue Reading

Citations

Dec 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A KayP M Hoogerbrugge
Sep 25, 2002·Korean Journal of Radiology : Official Journal of the Korean Radiological Society·Bo Yoon ChoiSeo Young Jeong
Nov 19, 2008·Journal of Drug Targeting·Hyun-Suk KangJeong-Sook Park
Dec 5, 2009·Journal of Drug Targeting·Lourena Mafra VerissimoE Sócrates Tabosa do Egito
Apr 26, 2014·Biomaterials Science·Archana N Rao, David W Grainger
Jul 7, 2005·Tissue Engineering·Sarwat F KhattakSusan C Roberts
Mar 11, 1999·Drug Development and Industrial Pharmacy·A VermeireJ P Remon
Mar 26, 2008·Chemical Communications : Chem Comm·Ruth LalorSusan E Matthews
Jan 22, 2011·Journal of Biomedical Materials Research. Part a·Yongzhuo HuangWenquan Liang
Mar 9, 2018·International Journal of Molecular Sciences·Ana Rey-Rico, Magali Cucchiarini
Oct 4, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Srikanth SriadibhatlaAlexander Kabanov
Aug 15, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tae Woo KimSeo Young Jeong
Jun 6, 2000·Advanced Drug Delivery Reviews·M C Woodle
Mar 15, 2001·Critical Reviews in Oncology/hematology·H H Sedlacek
Jun 3, 2004·Biomaterials·Woo-Jeong ChoiChong-Kook Kim
May 19, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshiyuki Hattori, Yoshie Maitani
Apr 26, 2005·International Journal of Pharmaceutics·Tae Woo KimSeo Young Jeong
Apr 22, 2006·Colloids and Surfaces. B, Biointerfaces·Yong-Zhuo HuangWen-Quan Liang
Feb 15, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tiffany Houchin-RayLonnie D Shea
May 22, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshiyuki HattoriYoshie Maitani
Oct 13, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Paola AstolfiMichela Pisani
Oct 24, 2014·The Journal of Physical Chemistry. B·Emilia FisicaroFrancesco Sansone
Aug 29, 2021·Pharmaceutics·José Antonio LebrónFrancisco J Ostos
Mar 30, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohamed MashalJose Luis Pedraz

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