Effect of nordihydroguaiaretic acid on cultured rat and human glioma cell proliferation

Journal of Neurosurgery
D E WilsonJ E Harris

Abstract

When cultured malignant cells derived from rat gliomas (C6 and 9L) and human gliomas (A-172 and T98G) were treated for 4 hours with 1 to 80 microns nordihydroguaiaretic acid (NDGA) or 5,8,11,14-eicosatetraynoic acid (ETYA), a dose-dependent inhibition of deoxyribonucleic acid (DNA) synthesis occurred. In a series of three experiments for each cell line, 40 microM NDGA suppressed 3H-thymidine incorporation in the rat and human glioma lines to an average of less than 3.1% and 5.6% of control uptake (counts per minute), respectively. Incubation with a higher concentration of ETYA (80 microM) resulted in inhibition of rat and human DNA synthesis to less than 53% and 62% of control levels, respectively. This inhibition was not associated with any loss of cell viability, as judged by trypan blue exclusion studies. Prolonged incubation (for 72 hours) of the rat and human glioma cells with NDGA markedly decreased cell proliferation with no loss of cell viability. The inhibition of human glioma cell division by NDGA was rapidly reversible after incubation for 24 hours and at least partially reversible after incubation for 96 hours. It is concluded that the inhibitors of eicosanoid biosynthesis, NDGA and (to a lesser extent) ETYA, reduce...Continue Reading

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Citations

Jun 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K M AndersonJ E Harris
Jul 1, 1992·Journal of the Neurological Sciences·W T CouldwellJ P Antel
Mar 3, 1998·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·A SpingarnS P Schantz
Dec 13, 2001·Neurochemistry International·Warren SuChung-Ren Jan
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Mar 22, 2016·Molecular Carcinogenesis·Simon K WrightFrank G Ondrey
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Oct 1, 1994·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K M AndersonJ E Harris
Oct 23, 2020·Journal of Ethnopharmacology·Monica Patricia Antonella CarabajalIris Catiana Zampini

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