Effect of Notch1 gene on remyelination in multiple sclerosis in mouse models of acute demyelination

Journal of Cellular Biochemistry
Hua FanXiao-Li Bai

Abstract

This study aims to explore the effects of Notch1 gene on remyelination in multiple sclerosis (MS). A mouse model of acute demyelination was successfully established and the model mice were grouped as cuprizone (CPZ) group, CPZ + small interfering RNA (siRNA)-Notch1 (siNotch1) group, and CPZ + siRNA negative control (NC) group. Meanwhile, another 3 groups (control, control + siNotch1, and control + siRNA NC) were established in normal mice. The changes of weight and maintenance time in rotating drum of mice were observed. Western blot analysis for the protein expressions related to Notch signaling pathway and oligodendrocyte (OL) differentiation in the corpus callosum of the mice. After model establishment, the weight of CPZ-induced demyelinated mice was decreased. During the repair period, the balance ability and movement of the mice was recovered, especially for those injected with siNotch1 plasmid. After model establishment, the number of myelinated axons was decreased. In comparison with the CPZ and CPZ siRNA NC groups, the CPZ + siNotch1 group had a decrease in the number of premature OLs, but increase in mature OLs, and a decrease in oligodendrocyte precursor cells and astrocytes. The expressions of proteins related to Not...Continue Reading

References

Nov 18, 2005·Journal of Neuroimmunology·Maciej JurynczykKrzysztof Selmaj
Jan 6, 2007·Organic & Biomolecular Chemistry·Chuanzheng ZhouJyoti Chattopadhyaya
Sep 18, 2007·Nature Medicine·Teresa PalomeroAdolfo A Ferrando
Oct 22, 2008·Nature Reviews. Neuroscience·Robin J M Franklin, Charles Ffrench-Constant
Oct 31, 2008·Lancet·Alastair Compston, Alasdair Coles
Sep 15, 2009·Journal of Neuroimmunology·Maciej Juryńczyk, Krzysztof Selmaj
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yueting ZhangGareth R John
Jan 5, 2010·Experimental Neurology·Stephen P J FancyRobin J M Franklin
Dec 7, 2010·Nature Neuroscience·Jeffrey K HuangRobin J M Franklin
Mar 30, 2011·FEBS Letters·Khalid A Hanafy, Jacob A Sloane
Aug 11, 2011·Development·Emma R AnderssonUrban Lendahl
Apr 3, 2012·The Journal of Clinical Investigation·Alyssa Nylander, David A Hafler
Mar 13, 2013·Cellular and Molecular Life Sciences : CMLS·Jean-Leon ThomasKari Alitalo
Aug 13, 2013·PloS One·Zaohua HuangAlexander I Agoulnik
Sep 17, 2013·Journal of Neurochemistry·Evangelina AparicioAna María Adamo
Jan 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hongfang WangJon C Aster
Feb 11, 2014·Neuron·Timothy R HammondVittorio Gallo
Mar 8, 2014·Journal of Neurochemistry·Veronique E Miron, Robin J M Franklin
Jul 19, 2014·Clinical and Experimental Medicine·Daniela MurtasMaria Teresa Perra
Oct 1, 2013·Biomolecular Concepts·Patricia MathieuLaura Morelli
Jun 20, 2015·PloS One·Galina DvoriantchikovaDmitry Ivanov
Oct 17, 2015·Methods in Molecular Biology·Thomas C RobertsMarc S Weinberg
Oct 31, 2015·Experimental Neurology·Ana Karolina de Santana NunesChristina Alves Peixoto
Mar 10, 2016·Experimental Neurology·Jenna M Gaesser, Sharyl L Fyffe-Maricich
Mar 12, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Dallas A Vanorny, Kelly E Mayo

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Citations

May 14, 2019·Expert Review of Neurotherapeutics·Brandon WilbanksMoses Rodriguez
Sep 11, 2019·Wiley Interdisciplinary Reviews. Developmental Biology·Diana M HoAngeliki Louvi
Jul 8, 2020·Biomolecules·Jose L SalazarShinya Yamamoto

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