PMID: 3747739Jul 1, 1986Paper

Effect of nutritional status on the fatty acid composition of rat liver and cultured hepatocytes

Lipids
G J NelsonJ E Hunt

Abstract

The lipid concentration and fatty acid composition of the whole liver and of cultured hepatocytes isolated from the livers of rats fed ad libitum (fed), fasted for 24 hr (fasted), or fasted for 48 hr and then refed a fat-free, high carbohydrate diet for 48 hr (refed) was studied. Hepatocytes were maintained as monolayer cultures in serum-free, lipid-free media and their fatty acid composition was analyzed at 3, 24, 48, 72 and 96 hr. The livers of fed animals, as well as their hepatocytes, contained less total lipid than those from animals on either of the other dietary regimes. Livers of fasted animals had three times the amount of lipid found in the livers of fed animals, and the livers of refed animals contained five times the amount of lipid as the livers of fed animals (all based on mg lipid/g wet weight of liver). The fatty acid composition of hepatocytes after 3 hr of culturing was very similar to that of fresh liver when compared in each of the dietary regimes. However, while the fatty acid compositions of livers and hepatocytes from fed and fasted animals were similar, the pattern in liver of refed animals was quite distinct from that of the fed animals. In the fed and fasted animals palmitic acid (16:0), stearic acid (...Continue Reading

References

Aug 1, 1979·The Journal of Nutrition·H SuzukiM Imamura
Mar 8, 1974·Molecular and Cellular Biochemistry·R R Brenner
Jun 1, 1973·The Journal of Nutrition·P S WadhwaD J Pringle
Dec 1, 1980·Diabetes·M J GeelenS A McCune
Jan 1, 1983·Annual Review of Biochemistry·S J WakilV C Joshi
Jan 1, 1983·The Journal of Nutrition·B S Alam, S Q Alam
Jan 1, 1980·Annals of the New York Academy of Sciences·A IchiharaH Shinno

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Citations

Jun 19, 2004·Journal of Toxicology and Environmental Health. Part a·Michael RubyBryce Landenberger
Nov 1, 1995·Metabolism: Clinical and Experimental·T MatsuoM Suzuki
Jan 1, 1997·Metabolism: Clinical and Experimental·T Matsuo, M Suzuki

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