Effect of oil overlay on inhibition potential of roscovitine in sheep cumulus-oocyte complexes

Reproduction in Domestic Animals = Zuchthygiene
L F CrocomoS D Bicudo

Abstract

Inhibitors of cyclin-dependent kinases, as roscovitine, have been used to prevent the spontaneous resumption of meiosis in vitro and to improve the oocyte developmental competence. In this study, the interference of oil overlay on the reversible arrest capacity of roscovitine in sheep oocytes as well as its effects on cumulus expansion was evaluated. For this, cumulus-oocyte complexes (COCs) were cultured for 20 h in TCM 199 with 10% foetal bovine serum (Control) containing 75 μm roscovitine (Rosco). Subsequently, they were in vitro matured (IVM) for further 18 h in inhibitor-free medium with LH and FSH. The culture was performed in Petri dishes under mineral oil (+) or in 96 well plates without oil overlay (-) at 38.5°C and 5% CO2 . At 20 and 38 h, the cumulus expansion and nuclear maturation were evaluated under stereomicroscope and by Hoechst 33342 staining, respectively. No group presented cumulus expansion at 20 h. After additional culture with gonadotrophins, a significant rate of COCs from both Control groups (+/-) exhibited total expansion while in both Rosco groups (+/-) the partial expansion prevailed. Among the oocytes treated with roscovitine, 65.2% were kept at GV in the absence of oil overlay while 40.6% of them r...Continue Reading

References

Oct 1, 1988·Biology of Reproduction·M A SirardN L First
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·John J EppigFrank L Pendola
Oct 4, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·M ShimadaT Terada
Jan 28, 2003·Molecular Reproduction and Development·Patrick LonerganTrudee Fair
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Lisa M Mehlmann
Mar 8, 2006·Reproduction in Domestic Animals = Zuchthygiene·C RodríguezP de Paz
Jul 1, 1991·Theriogenology·M MattioliE Seren
Jul 1, 1993·Theriogenology·S NatsuyamaT Mori
Jun 8, 2006·Journal of Assisted Reproduction and Genetics·Jin Cheol TaeJin Ho Lim
Feb 10, 2007·Molecular Reproduction and Development·S SuccuS Naitana
Nov 27, 2008·Reproduction in Domestic Animals = Zuchthygiene·A ShiraziB Heidari
Apr 14, 2009·Fertility and Sterility·Wei ShaQing-Yuan Sun
Oct 31, 1935·The Journal of Experimental Medicine·G Pincus, E V Enzmann
Jun 24, 2010·Zygote : the Biology of Gametes and Early Embryos·Letícia Siqueira Sá BarrettoGisele Zoccal Mingoti

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Citations

Jul 15, 2015·Zygote : the Biology of Gametes and Early Embryos·Letícia Ferrari CrocomoSony Dimas Bicudo
Feb 16, 2019·Zygote : the Biology of Gametes and Early Embryos·A A A SilvaE S Lopes Júnior

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