Effect of oleic acid on store-operated calcium entry in immune-competent cells

European Journal of Nutrition
Celia CarrilloSara R Alonso-Torre

Abstract

To study the mechanism by which oleic acid (OA) (C18:1) exerts its beneficial effects on immune-competent cells. Since store-operated Ca(2+) entry (SOCE) is a Ca(2+) influx pathway involved in the control of multiple physiological processes including cell proliferation, we studied the effect of OA in Ca(2+) signals of Jurkat T cells and THP-1 monocytes, paying particular attention to SOCE. Changes in [Ca(2+)]i were measured using the Fura-2 fluorescence dye. Mn(2+) uptake was monitored as a rate of quenching of Fura-2 fluorescence measured at the Ca(2+)-insensitive wavelengths. Thapsigargin was used to induce SOCE in Fura-2-loaded cells. We showed a clear dose-dependent SOCE-inhibitory effect of OA in both cell lines. Such an inhibitory effect was PKC independent and totally restored by albumin, suggesting that OA exerts its effect somewhere in the membrane. We also demonstrated that OA induces increases in [Ca(2+)]i partly mediated by an extracellular Ca(2+) influx through econazole-insensitive channels. Finally, we compared the effect of OA with stearic acid (C18:0), assuming the emerged evidence concerning the link between saturated fats and inflammation disorders. Stearic acid failed to inhibit SOCE, independently on the co...Continue Reading

References

Feb 15, 1992·The Biochemical Journal·S G Chen, K Murakami
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Feb 1, 1986·Cell Calcium·J W Putney
Jul 14, 1989·Biochemical and Biophysical Research Communications·M T AlonsoJ García-Sancho
Jun 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J Berridge
Oct 17, 1994·FEBS Letters·A B Khodorova, E I Astashkin
Jul 1, 1994·Molecular and Cellular Endocrinology·E EkokoskiK Törnquist
Oct 1, 1995·Pflügers Archiv : European journal of physiology·C Villalobos, J García-Sancho
Oct 7, 1997·Biochimica Et Biophysica Acta·S R Alonso-Torre, J García-Sancho
Jan 13, 1998·Biochemical and Biophysical Research Communications·A GamberucciA Benedetti
Dec 13, 2003·Endocrine Reviews·Christina R Kahl, Anthony R Means
Mar 25, 2005·Physiological Reviews·Anant B Parekh, James W Putney
Dec 13, 2005·The Journal of Physiology·Lucía NúñezCarlos Villalobos
Jun 17, 2008·British Journal of Pharmacology·H Meves
Sep 16, 2009·Annals of Nutrition & Metabolism·Claudio Galli, Philip C Calder
Feb 2, 2011·The Journal of Rheumatology. Supplement·Hani El-GabalawyCharles N Bernstein
Apr 8, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Celia CarrilloSara R Alonso-Torre
May 18, 2011·Biochemical and Biophysical Research Communications·Maria A HidalgoRafael A Burgos
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Celia CarrilloSara R Alonso-Torre
Oct 1, 2011·European Journal of Nutrition·Celia CarrilloSara R Alonso-Torre
Feb 7, 2012·Biochimica Et Biophysica Acta·Celia CarrilloNaim Akhtar Khan
Jan 3, 2013·Mini Reviews in Medicinal Chemistry·Helioswilton Sales-CamposCristina Ribeiro Cardoso
Nov 10, 2013·Pharmacology & Therapeutics·Clara M YatesG Ed Rainger
Nov 29, 2013·Annals of the Rheumatic Diseases·Klaus W FrommerElena Neumann
Jan 30, 2014·American Journal of Respiratory Cell and Molecular Biology·Michael A ThompsonChristina M Pabelick
Feb 18, 2014·Pflügers Archiv : European journal of physiology·Jean CheminPhilippe Lory
Aug 15, 2014·Critical Reviews in Food Science and Nutrition·Javier Delgado-ListaJose Lopez-Miranda
Sep 6, 2014·Nutrients·Elena García-FernándezAnna Bach-Faig

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.