Effect of ompR gene mutation in expression of ompC and ompF of Salmonella typhi

Interdisciplinary Sciences, Computational Life Sciences
Mahaboobbasha MalickbashaGurusamy Annadurai

Abstract

In the present investigation, a total of 50 stool samples were collected from the food handlers to screen the typhoid asymptomatic carriers, positive result was yielded for 2 out of the 50 samples. Salmonella typhi was isolated and identified based on the cultural characteristics on BSA, Macconkey agar, XLD and phylogenetic analysis. The ompR region of these two strains was amplified, sequenced and a Neighbor-Joining algorithm tree of ompR was constructed. The isolates were designated as (Salmonella Strain) SS-3 and SS-5 respectively. The isolates were subjected to mutation using sodium chloride at various osmolarity concentrations in LB broth. Both the wild and mutant Salmonella typhi were used for the isolation of outer membrane protein. The outer membrane protein was isolated and compared with both the wild and mutated Salmonella typhi. The expression of outer membrane protein was showing the variation in the expression which were noticed by using SDS-PAGE. On the basis of the results, it was concluded that the ompR-envZ two component regulatory systems play an important role on the regulation of Vi polysaccharide synthesis in S. typhi, and that one of the environmental signals for this regulation may be osmolarity.

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Citations

Jun 26, 2013·Biologicals : Journal of the International Association of Biological Standardization·Hoda ToobakShakiba Darvish Alipour Astaneh
Nov 26, 2015·Archives of Microbiology·X Vega-ManriquezA Verdugo-Rodríguez
Oct 22, 2011·Asian Pacific Journal of Tropical Medicine·Balaji Chandrasekaran, Senthilkumar Balakrishnan
May 30, 2020·World Journal of Microbiology & Biotechnology·Aqsa ShaheenMoazur Rahman
Jul 2, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Robab AzargunShirin Eyvazi
Jan 10, 2017·Frontiers in Microbiology·Piotr MajewskiElżbieta Tryniszewska

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