PMID: 19912859Jun 1, 1992Paper

Effect of opioid receptor antagonism on proopiomelanocortin peptide levels and gene expression in the hypothalamus

Molecular and Cellular Neurosciences
C E MarkowitzS L Wardlaw

Abstract

In order to determine how brain beta-endorphin (beta-EP) and its precursor proopiomelanocortin (POMC) adapt to chronic opioid blockade we have examined the effects of treatment with the opioid receptor antagonist naltrexone (NTX) on POMC gene expression and peptide levels in the hypothalamus. Male rats were treated with NTX by daily injection or constant minipump infusion. RNA was isolated from the medial basal hypothalamus (MBH) after an aliquot was removed for peptide RIA and the amount of POMC mRNA was measured by solution hybridization SI nuclease protection assay. beta-EP and several other POMC-derived peptides including alpha-melanocyte-stimulating hormone (alpha-MSH) and corticotropin-like intermediate lobe peptide (CLIP) or gamma(3)-MSH were measured in the MBH and anterior hypothalamus (AH) by RIA. In an initial experiment POMC peptide levels were measured after 7 days of NTX (4.8 mg/day) infusion. There was a marked fall in the concentrations of beta-EP, alpha-MSH, and CLIP; levels in the MBH declined by more than 60% (P < 0.001). In the next experiment NTX (1 mg) was injected daily and POMC peptides and mRNA were measured after 2 and 5 days of treatment. (beta-EP) and alpha-MSH levels fell progressively in the MBH an...Continue Reading

References

Sep 15, 1978·European Journal of Pharmacology·R A Lahti, R J Collins
Jun 15, 1985·American Journal of Obstetrics and Gynecology·S S YenN S Cetel
Jun 1, 1987·The Journal of Clinical Endocrinology and Metabolism·L Wildt, G Leyendecker
Apr 1, 1982·The Journal of Clinical Endocrinology and Metabolism·J EllingboeN K Mello
Jul 1, 1980·The Journal of Clinical Endocrinology and Metabolism·M E Quigley, S S Yen

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Citations

Feb 15, 2012·Molecular Neurobiology·Masoud Ghamari-Langroudi
Sep 5, 1996·Brain Research. Molecular Brain Research·S L WardlawS Sobieszczyk
Jul 28, 2015·Pharmacology & Therapeutics·Kimberley A Pitman, Stephanie L Borgland
Nov 29, 2016·American Journal of Physiology. Endocrinology and Metabolism·Gabrielle Page-WilsonSharon L Wardlaw
Dec 28, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Mauro CataldiAnnamaria Colao
Jul 11, 2012·Endocrinology·Roxanne DutiaSharon L Wardlaw
Dec 22, 2017·Journal of the Endocrine Society·Rebecca J GordonSharon L Wardlaw
Mar 2, 2019·Journal of the Endocrine Society·Sunil K PanigrahiSharon L Wardlaw
May 1, 1999·Journal of Neuroendocrinology·A D Papadopoulos, S L Wardlaw

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