Effect of orally administered probiotic E. coli strain Nissle 1917 on intestinal mucosal immune cells of healthy young pigs

Veterinary Immunology and Immunopathology
S DunckerStephan C Bischoff

Abstract

Several beneficial effects of probiotics have been described in studies using rodent disease models and in human patients; however, the underlying mechanisms remained mostly unclear. Only a few studies focused on the effects of probiotics on the intestinal mucosal immune system. Here, we studied the effect of the probiotic strain E. coli Nissle 1917 (EcN) administered orally to young pigs at two concentrations (10(9) and 10(11)CFU/d for 21 days) on the gut-associated lymphatic tissue. This probiotic strain was shown recently to reduce recurrence of inflammation in ulcerative colitis patients. We quantified the number and distribution of intestinal immune cells (granulocytes, mast cells, CD4+, CD8+, CD25+, IgA+ lymphocytes) and the mucosal mRNA expression of cytokines (IFN-gamma, TNF-alpha, TGF-beta, IL-10) and antimicrobial peptides (PR-39, NK-lysin, prepro-defensin-beta 1, protegrins). The number and distribution of cells were highly different between small intestinal and colon segments in all groups, but were not influenced by EcN, except high dose EcN fed pigs (10(11) CFU/d) showing an increase in mucosal CD8+ cells in the ascending colon. The mRNA analysis revealed no changes associated with EcN feeding. In conclusion, acco...Continue Reading

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Citations

Mar 27, 2007·Applied and Environmental Microbiology·Sandra Da ReChristophe Beloin
Feb 26, 2011·World Journal of Gastroenterology : WJG·Jan BuresMarcela Kopacova
Jun 27, 2012·BMC Veterinary Research·Cheng SuoQing Gu
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May 15, 2007·Veterinary Immunology and Immunopathology·Peter SchierackLydia Scharek
Oct 21, 2015·International Immunopharmacology·Lifeng WangHeping Zhang
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Oct 1, 2009·Archives of Animal Nutrition·Lydia Scharek-TedinMichael F G Schmidt
May 7, 2020·Veterinary Medicine International·Daiga GāliņaAnda Valdovska

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