Effect of organic matter on the in vitro photoeradication of Pseudomonas aeruginosa by means of a cationic tetraaryl-porphyrin

Photochemistry and Photobiology
Viviana T OrlandiPaola Barbieri

Abstract

Photodynamic therapy is emerging as an antimicrobial alternative approach; the concomitant presence of a photosensitizer (PS), O(2) and visible light induces lethal oxidative damages to bacterial cells. Among Gram-negative bacteria, Pseudomonas aeruginosa seems to be one of the least susceptible to photodynamic treatment. In this study, we evaluated the influence of several experimental conditions on photoeradication of a planktonic culture of P. aeruginosa PAO1 by means of a tetracationic meso-arylsubstituted porphyrin (RM24). Our findings suggest that the photo-oxidative stress induced by RM24 is strictly correlated to the amount of PS bound to the cells that in turn decreases with the increasing concentrations of organic compounds in the medium. The photoeradication is dependent on PS concentrations, cellular density and light dose. RM24 was able to induce oxidative stress by means of singlet oxygen formation, although ROS formation cannot be ruled out. The standardized experimental conditions of the photospot test allowed us to evidence intraspecific PDT sensitivity differences among three strains of P. aeruginosa.

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Citations

Sep 18, 2013·Journal of Photochemistry and Photobiology. B, Biology·Viviana T OrlandiPaola Barbieri
Nov 4, 2015·International Journal of Molecular Sciences·Ruoxian DengXiaofeng Jia
Nov 17, 2015·International Journal of Molecular Sciences·Rubén Ruiz-GonzálezSanti Nonell
Sep 26, 2013·Current Opinion in Pharmacology·Rui YinMichael R Hamblin
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Rahmatollah RahimiMehdi Rassa
May 13, 2015·Journal of Photochemistry and Photobiology. B, Biology·Kristine Opsvik WikeneHanne Hjorth Tønnesen
Mar 7, 2017·Future Medicinal Chemistry·Lúcia MarcielAdelaide Almeida
Nov 6, 2020·Photochemistry and Photobiology·Fatemeh FayyazRahmatollah Rahimi

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