Effect of organic solvents on normal human stratum corneum: evaluation by the corneoxenometry bioassay

Dermatology : International Journal for Clinical and Investigative Dermatology
V GoffinG E Piérard

Abstract

Organic solvents alter the stratum corneum structure and barrier function. To measure the effect of various solvents upon human stratum corneum using the ex vivo corneoxenometry bioassay which is a variant of corneosurfametry. Corneoxenometry entails collection of human stratum corneum by cyanoacrylate. The material is immersed in organic solvents for periods ranging from 1 to 120 min. After staining the samples with a toluidine blue-basic fuchsin solution, the color is measured using reflectance colorimetry. Solvent aggressivity to the stratum corneum correlates with the color darkening of the samples. The least aggressive solvent was hexane, followed by ethanol, methanol, hexane-ethanol, chloroform, chloroform-methanol and hexane-methanol. The influence of contact time between solvents and the stratum corneum showed a logarithmic pattern which varied according to the solvent. Data are in line with previous experiments conducted in vivo and in vitro, thus indicating the predictive value of corneoxenometry. Such a bioassay may avoid hazards of some in vivo human testings.

Citations

Apr 20, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·G PielB Evrard
Aug 24, 1999·Clinical and Experimental Dermatology·V GoffinG E Piérard
Jul 29, 2008·Journal of Occupational Medicine and Toxicology·Emmanuelle Xhauflaire-UhodaGérald E Piérard
Sep 16, 2008·Journal of Cosmetic Dermatology·Emmanuelle Xhauflaire-UhodaGérald E Piérard
Apr 20, 2007·Contact Dermatitis·Pascale QuatresoozGérald E Piérard
Sep 2, 2014·TheScientificWorldJournal·Gérald E PiérardPhilippe Delvenne

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